Pyridostatin hydrochloride (Synonyms: RR82 hydrochloride) |
| カタログ番号GC37043 |
ピリドスタチン (RR82) 塩酸塩は、G-四重鎖 DNA 安定剤です (Kd = 490 nM)。ピリドスタチン塩酸塩は、複製および転写依存の DNA 損傷を誘発することにより、ヒト癌細胞の増殖停止を促進します。ピリドスタチン塩酸塩は、がん原遺伝子 Src を標的とします。ピリドスタチン塩酸塩は、ヒト乳癌細胞の SRC タンパク質レベルと SRC 依存性細胞運動性を低下させました。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1781882-65-2
Sample solution is provided at 25 µL, 10mM.
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Pyridostatin hydrochloride is a G-quadruplexe stabilizer, with a Kd of 490 nM. Kd: 490 nM (G-quadruplexe)[1]
Pyridostatin hydrochloride is a G-quadruplexe stabilizer, with a Kd of 490 nM[1]. Pyridostatin (PDS) shows neurotoxic activity against primary cortical neurons at 0.01-5 μM, causes DNA double-strand breaks (DSBs) at 1 μM, downregulates BRCA1 in neurons at 1, 2 or 5 μM[2]. Pyridostatin interacts with G-quadruplex motifs in SRC and alters mRNA levels of damaged genes[3].
[1]. Koirala D, et al. A single-molecule platform for investigation of interactions between G-quadruplexes and small-molecule ligands. Nat Chem. 2011 Aug 28;3(10):782-7. [2]. Moruno-Manchon JF, et al. The G-quadruplex DNA stabilizing drug pyridostatin promotes DNA damage and downregulates transcription of Brca1 in neurons. Aging (Albany NY). 2017 Sep 12;9(9):1957-1970. [3]. Rodriguez R, et al. Small-molecule-induced DNA damage identifies alternative DNA structures in human genes. Nat Chem Biol. 2012 Feb 5;8(3):301-10.
| Cas No. | 1781882-65-2 | SDF | |
| 同義語 | RR82 hydrochloride | ||
| Canonical SMILES | O=C(C1=CC(OCCN)=CC(C(NC2=NC3=C(C(OCCN)=C2)C=CC=C3)=O)=N1)NC4=CC(OCCN)=C5C=CC=CC5=N4.Cl.Cl.Cl.Cl.Cl | ||
| Formula | C31H37Cl5N8O5 | M.Wt | 778.94 |
| 溶解度 | Water: 50 mg/mL (64.19 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.2838 mL | 6.419 mL | 12.838 mL |
| 5 mM | 256.8 μL | 1.2838 mL | 2.5676 mL |
| 10 mM | 128.4 μL | 641.9 μL | 1.2838 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)
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