HMBR |
カタログ番号GC66439
One-Click Copy Product Info
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HMBRは非蛍光性で、細胞透過性蛍光生成染料であり、黄色蛍光活性化と吸収波長シフト型タグ（Y-FAST）に結合し、青色光の励起後に黄色い蛍光を放ち、Ex/Em値は491/525-539nmである。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1287651-36-8
Sample solution is provided at 25 µL, 10mM.
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HMBRは非蛍光性で、細胞透過性蛍光生成染料であり、黄色蛍光活性化と吸収波長シフト型タグ（Y-FAST）に結合し、青色光の励起後に黄色い蛍光を放ち、Ex/Em値は491/525-539nmである[1,2]。HMBRは低い細胞毒性を示し、よく生細胞（ゼブラフィッシュの胚など）におけるタンパク質発現の検出とイメージング用の蛍光レポーターとして使われる[3,4,5]。
HMBRによる生細胞のイメージング[1]：
（1）核H2B-FASTを発現するHeLa細胞をPBSで洗浄し、その後は5μM HMBRを含有するDMEM培地（血清、フェノールレッドなし）で処理した。
（2）洗浄なしに細胞を直接イメージングした。
（3）Plan Apochromat 63×/1.4 NA油浸対物レンズを搭載したZeiss LSM 710レーザー走査型共焦点顕微鏡を使い、イメージングを行った。
（4）488nmの励起波長、493から575nmの発光波長で、緑色のチャネルでFAST:HMBRのイメージングを行った。
| Cas No. | 1287651-36-8 | SDF | |
| Formula | C11H9NO2S2 | M.Wt | 251.32 |
| 溶解度 | DMSO : 125 mg/mL (497.37 mM; ultrasonic and warming and heat to 80°C) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.979 mL | 19.895 mL | 39.7899 mL |
| 5 mM | 795.8 μL | 3.979 mL | 7.958 mL |
| 10 mM | 397.9 μL | 1.9895 mL | 3.979 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)