HMBR |
Catalog No.GC66439
One-Click Copy Product Info
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HMBR is a non-fluorescent, cell-permeable fluorogenic dye that binds to the Yellow Fluorescence-Activating and Absorption-Shifting Tag (Y-FAST) and emits yellow fluorescence upon blue light excitation, with Ex/Em of 491/525-539nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1287651-36-8
Sample solution is provided at 25 µL, 10mM.
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HMBR is a non-fluorescent, cell-permeable fluorogenic dye that binds to the Yellow Fluorescence-Activating and Absorption-Shifting Tag (Y-FAST) and emits yellow fluorescence upon blue light excitation, with Ex/Em of 491/525-539nm[1,2]. HMBR exhibits low cytotoxicity and is commonly used as a fluorescent reporter for detecting and imaging protein expression in living cells, such as in zebrafish embryos[3,4,5].
Reference:
[1] Plamont M A, Billon-Denis E, Maurin S, et al. Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo[J]. Proceedings of the National Academy of Sciences, 2016, 113(3): 497-502.
[2] Pimenta F M, Chiappetta G, Le Saux T, et al. Chromophore renewal and fluorogen-binding tags: a match made to last[J]. Scientific Reports, 2017, 7(1): 12316.
[3] Tebo A G, Moeyaert B, Thauvin M, et al. Orthogonal fluorescent chemogenetic reporters for multicolor imaging[J]. Nature Chemical Biology, 2021, 17(1): 30-38.
[4] Streett H E, Kalis K M, Papoutsakis E T. A strongly fluorescing anaerobic reporter and protein-tagging system for Clostridium organisms based on the fluorescence-activating and absorption-shifting tag protein (FAST)[J]. Applied and Environmental Microbiology, 2019, 85(14): e00622-19.
[5] El Hajji L, Bunel B, Joliot O, et al. A tunable and versatile chemogenetic near-infrared fluorescent reporter[J]. Nature Communications, 2025, 16(1): 2594.
Use of HMBR for live-cell imaging[1]:
(1) HeLa cells expressing nuclear H2B-FAST were washed with PBS and then treated with DMEM medium (without serum and phenol red) containing 5μM HMBR.
(2) Cells were imaged directly without washing.
(3) Imaging was performed using a Zeiss LSM 710 laser scanning confocal microscope equipped with a Plan Apochromat 63×/1.4 NA oil immersion objective.
(4) FAST:HMBR was imaged using the green channel with excitation at 488nm and emission detection from 493 to 575nm.
Reference:
[1] Li C, Plamont M A, Sladitschek H L, et al. Dynamic multicolor protein labeling in living cells[J]. Chemical Science, 2017, 8(8): 5598-5605.
| Cas No. | 1287651-36-8 | SDF | |
| Formula | C11H9NO2S2 | M.Wt | 251.32 |
| Solubility | DMSO : 125 mg/mL (497.37 mM; ultrasonic and warming and heat to 80°C) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.979 mL | 19.895 mL | 39.7899 mL |
| 5 mM | 795.8 μL | 3.979 mL | 7.958 mL |
| 10 mM | 397.9 μL | 1.9895 mL | 3.979 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)