HMBR |
Catalog No.GC66439
One-Click Copy Product Info
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HMBR은 무형광성 세포 투과성 형광 염료로 옐로우 플루오레스센스-활성화 및 흡수 이동 태그 (Y-FAST)에 결합해서 청색광 여기 시 노란색 형광을 방출하고 Ex/Em은 491/525-539nm이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1287651-36-8
Sample solution is provided at 25 µL, 10mM.
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HMBR은 무형광성 세포 투과성 형광 염료로 옐로우 플루오레스센스-활성화 및 흡수 이동 태그(Y-FAST)에 결합해서 청색광 여기 시 노란색 형광을 방출하고 Ex/Em은 491/525-539nm이다. HMBR은 낮은 세포 독성을 나타내고 생세포에서 단백질 발현을 검출하고 이미징하기 위한 형광 리포터로 일반적으로 사용된다. 예를 들어 제브라피쉬 배아에서 사용된다[3,4,5].
Reference:
[1] Plamont M A, Billon-Denis E, Maurin S, et al. Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo[J]. Proceedings of the National Academy of Sciences, 2016, 113(3): 497-502.
[2] Pimenta F M, Chiappetta G, Le Saux T, et al. Chromophore renewal and fluorogen-binding tags: a match made to last[J]. Scientific Reports, 2017, 7(1): 12316.
[3] Tebo A G, Moeyaert B, Thauvin M, et al. Orthogonal fluorescent chemogenetic reporters for multicolor imaging[J]. Nature Chemical Biology, 2021, 17(1): 30-38.
[4] Streett H E, Kalis K M, Papoutsakis E T. A strongly fluorescing anaerobic reporter and protein-tagging system for Clostridium organisms based on the fluorescence-activating and absorption-shifting tag protein (FAST)[J]. Applied and Environmental Microbiology, 2019, 85(14): e00622-19.
[5] El Hajji L, Bunel B, Joliot O, et al. A tunable and versatile chemogenetic near-infrared fluorescent reporter[J]. Nature Communications, 2025, 16(1): 2594.
HMBR을 생세포 이미징에 사용[1]:
(1) 핵 H2B-FAST를 발현하는 HeLa 세포를 PBS로 세척한 후 5μM HMBR이 포함된 DMEM 배양지(혈청 및 페놀레드 없음)로 처리하였습니다.
(2) 세포를 세척하지 않고 직접 이미징하였습니다.
(3) Zeiss LSM 710 레이저 스캐닝 공초점 현미경을 사용해서 이미징을 수행하였고Plan Apochromat 63×/1.4 NA 오일 침지 대물렌즈가 장착되어 있었습니다.
(4) FAST:HMBR은 488nm에서 여기하고 493에서 575nm까지 방출을 검출하는 녹색 채널을 사용해서 이미징하였습니다.
Reference:
[1] Li C, Plamont M A, Sladitschek H L, et al. Dynamic multicolor protein labeling in living cells[J]. Chemical Science, 2017, 8(8): 5598-5605.
| Cas No. | 1287651-36-8 | SDF | |
| Formula | C11H9NO2S2 | M.Wt | 251.32 |
| Solubility | DMSO : 125 mg/mL (497.37 mM; ultrasonic and warming and heat to 80°C) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.979 mL | 19.895 mL | 39.7899 mL |
| 5 mM | 795.8 μL | 3.979 mL | 7.958 mL |
| 10 mM | 397.9 μL | 1.9895 mL | 3.979 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)