MTT (Synonyms: Methylthialazole Tetrazolium, NSC 60102, Thiazolyl Blue, Thiazolyl Blue Tetrazolium Bromide) |
| カタログ番号GC14731 |
MTT（MTT）は、細胞増殖を測定するために広く使用されている比色試薬です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 298-93-1
Sample solution is provided at 25 µL, 10mM.
MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) is a reagent used in the measurement of in vitro cell proliferation.
Tetrazolium salts have been the most widely used tools in cell biology for deternining the metabolic activity of cells ranging from microbial origin to mammalian. Fractionation studies in mammalian cells indicate that NADH, the reduced pyridine nucleotide cofactor, is responsible for most MTT reduction, which is further supported by studies with whole cells.
In vitro: It has been found that MTT reduction was associated not only with mitochondria, but also with the cytoplasm and nonmitochondrial membranes including endosome/lysosome compartment and plasma membrane. The net positive charge on MTT appears to be the predominant factor involved in their cellular uptake through the potential of plasma membrane. However, the second generation tetrazolium dyes (XTT, WST-1 and to some extent, MTS) that form water-soluble formazans and require an intermediate electron acceptor for reduction, are characterised by a net negative charge and therefore are largely cell-impermeable [1].
In vivo: MTT currently is only used for in vitro cell proliferation determination and there is no in vivo study reported.
Reference:
[1] Berridge MV,Herst PM,Tan AS. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev.2005;11:127-52.
| Cas No. | 298-93-1 | SDF | |
| 同義語 | Methylthialazole Tetrazolium, NSC 60102, Thiazolyl Blue, Thiazolyl Blue Tetrazolium Bromide | ||
| Chemical Name | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide | ||
| Canonical SMILES | CC1=C(C)N=C([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)S1.[Br-] | ||
| Formula | C18H16N5SBr | M.Wt | 414.32 |
| 溶解度 | ≥ 41.4mg/mL in DMSO | Storage | Store at 2-8°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4136 mL | 12.068 mL | 24.1359 mL |
| 5 mM | 0.4827 mL | 2.4136 mL | 4.8272 mL |
| 10 mM | 0.2414 mL | 1.2068 mL | 2.4136 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
A. The cytotoxicity of R-CDs.
Next, 10 μL of MTT(GlpBio) with the concentration of 100 μg⋅mL− 1 was separately added into each well and another 4 h incubation was conducted.
Spectrochim Acta A 281 (2022): 121581. PMID: 35797950 IF: 4.8311 -
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Subsequently, the treated cells were stained 2 BioMed Research International with 10 μl MTT (5 mg/ml) (Glpbio) and hatched sequentially at 37°C for another 4 h.
BioMed Research International 2020 (2020). PMID: 32090104 IF: 3.414 -
Related Biological Data
TLC analysis of EA-BLR24 and bioautography. Revealed with 365 nm UV light (A) and iodine vapor (B). Zones of inhibition by the bioactive fraction on TLC plate (C) and dye by MTT (D)
After incubation, MTT solution (0.5 mg/mL, GlpBio) was sprayed over the plate, coloring reacting at 37°C for 30 min.
Braz J Microbiol (2024): 1-14. PMID: 38261262 IF: 2.2
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