Myriocin (Synonyms: ISP-1; Thermozymocidin) |
カタログ番号GC14278
One-Click Copy Product Info
|
免疫抑制剤および特定のセリンパルミトイルトランスフェラーゼ阻害剤
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 35891-70-4
Sample solution is provided at 25 µL, 10mM.
Myriocin is an amino fatty acid antibiotic derived from certain thermophylic fungi, in this case Mycelia sterilia. Myriocin is a potent immunosuppressant having 10- to 100-fold more activity than cyclosporin A.[1] Myriocin is a potent inhibitor of serine palmitoyltransferase (Ki = 0.28 nM), the enzyme that catalyzes the first step of sphingolipid biosynthesis. [2] Myriocin disrupts substratum adhesion of melanoma cells.[3] Myriocin also suppresses cell proliferation in the murine cytotoxic T cell line CTLL-2 (IC50 = 15 nM) via apoptosis.[2],[4] Myriocin suppresses replication of the hepatitis C virus in a murine model.[5]
Reference:
[1]. Fujita, T., Inoue, K., Yamamoto, S., et al. Fungal metabolites. Part 11. A potent immunosuppressive activity found in Isaria sinclairii metabolite. Journal of Antibiotics 47, 208-215 (1994).
[2]. Miyake, Y., Kozutsumi, Y., Nakamura, S., et al. Serine palmitoyltransferase is the primary target of a sphingosine-like immunosuppressant, ISP-1/myriocin. Biochemical and Biophysical Research Communications 211(2), 396-403 (1995).
[3]. Hidari, K.I.P.J., Ichikawa, S., Fujita, T., et al. Complete removal of sphingolipids from the plasma membrane disrupts cell to substratum adhesion of mouse melanoma cells. The Journal of Biological Chemisty 271(24), 14636-14641 (1996).
[4]. Nakamura, S., Kozutsumi, Y., Sun, Y., et al. Dual roles of sphingolipids in signaling of the escape from and onset of apoptosis in a mouse cytotoxic T-cell line, CTLL-2. The Journal of Biological Chemisty 271, 1255-1257 (1996).
[5]. Umehara, T., Sudoh, M., Yasui, F., et al. Serine palmitoyltransferase inhibitor suppresses HCV replication in a mouse model. Biochemical and Biophysical Research Communications 346, 67-73 (2006).
| Cas No. | 35891-70-4 | SDF | |
| 同義語 | ISP-1; Thermozymocidin | ||
| Chemical Name | (2S,3R,4R,E)-2-amino-3,4-dihydroxy-2-(hydroxymethyl)-14-oxoicos-6-enoic acid | ||
| Canonical SMILES | CCCCCCC(CCCCCC/C([H])=C([H])/C[C@](O)([H])[C@@](O)([H])[C@@](C(O)=O)(N)CO)=O | ||
| Formula | C21H39NO6 | M.Wt | 401.54 |
| 溶解度 | Methanol: 2 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4904 mL | 12.4521 mL | 24.9041 mL |
| 5 mM | 498.1 μL | 2.4904 mL | 4.9808 mL |
| 10 mM | 249 μL | 1.2452 mL | 2.4904 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
-
Related Biological Data
Purified human SPT-ORMDL complexes can be inhibited by C6-ceramide. g Selective inhibition of SPT-ORMDL3 by C6 ceramide among various sphingolipid species or analogs. Data are presented as mean values ± SEM of three independent experiments. The different sphingolipid species or analogs were applied at 10μM in each assay.
C8-ceramide at indicated concentration or 1µM myriocin (GLPBIO, USA) for 1 hr at 37 °C
Nature Communications 14.1 (2023): 3475. PMID: 37308477 IF: 16.6009 -
Related Biological Data
Disturbed sphingolipid contents promote translocation of GFP-bZIP28 from the endoplasmic reticulum (ER) to the nucleus. (c) Detection of protein ubiquitination level after treatments.
Ten-day-old wild-type seedlings were transferred to liquid 1/2 MS andpretreated with 1μM myriocin (GLPBIO, USA) or DMSO for 3h.
New Phytologist (2023). PMID: 37606093 IF: 9.4006 -
Related Biological Data
Downregulation of ceramide levels promotes PoRV replication. (D–E and H–I) Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I).
IPEC-J2 cells cultured in 6-cm dishes were infected with PoRV or treated with inhibitors (Myriocin (GLPBIO, USA), 20µM; Fumonisin B1, 50µM; Amitriptyline HCl, 30µM; or GW4869, 10µM) for several hours.
Journal of Virology (2024): e00064-24. PMID: 38488360 IF: 5.3999
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)