Nicotinamide (Vitamin B3) (Synonyms: Niacinamide, Nicotinic Acid Amide, NSC 13128, NSC 27452, Vitamin B3 Amide) |
カタログ番号GN10347
One-Click Copy Product Info
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ニコチンアミド(ビタミンB3)（Nicotinamide (Vitamin B3)）はビタミンB3のアミド誘導体であり、SIRT1 (IC50: 50-180 μM)とSIRT2 (IC50: 2 μM)の活性を阻害することができる。ニコチンアミドは細胞内のNAD+, ATPとROSの濃度を増やすこともできる。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 98-92-0
Sample solution is provided at 25 µL, 10mM.
ニコチンアミド(ビタミンB3)（Nicotinamide (Vitamin B3)）はビタミンB3のアミド誘導体であり、SIRT1 (IC50: 50-180 μM)とSIRT2 (IC50: 2 μM)の活性を阻害することができる。ニコチンアミドは細胞内のNAD+, ATPとROSの濃度を増やすこともできる[3]。
ニコチンアミド (5mM ,72時間)を老化した線維芽細胞に投与したところ、ROSレベルを減らし、老化線維芽細胞における老化表現型の出現を減少させる[1]。ニコチンアミド(50-200μM)でHepG2細胞を処理した結果、用量依存的にNAD+の生合成を促進した[4]。
ニコチンアミド(0.5/1.0 mg/kg)の処理は、最大の寿命を影響せずに、全身の生理機能と生体内の代謝を変えられる[2]。長期的なニコチンアミド(300mg/kg/日；六ヶ月間と十二ヶ月間の間)の投与は、末梢組織における年齢関係の遺伝子表現の変化を逆転させ、骨格筋のミトコンドリアの呼吸容量を高める[5]。
References:
[1]. Kwak JY, Ham HJ, Kim CM, Hwang ES. Nicotinamide exerts antioxidative effects on senescent cells. Mol Cells. 2015 Mar;38(3):229-35.
[2]. Mitchell SJ, Bernier M, Aon MA, Cortassa S, Kim EY, Fang EF, Palacios HH, Ali A, Navas-Enamorado I, Di Francesco A, Kaiser TA, Waltz TB, Zhang N, Ellis JL, Elliott PJ, Frederick DW, Bohr VA, Schmidt MS, Brenner C, Sinclair DA, Sauve AA, Baur JA, de Cabo R. Nicotinamide Improves Aspects of Healthspan, but Not Lifespan, in Mice. Cell Metab. 2018 Mar 6;27(3):667-676.e4.
[3].Hwang ES, Song SB. Nicotinamide is an inhibitor of SIRT1 in vitro, but can be a stimulator in cells. Cell Mol Life Sci. 2017 Sep;74(18):3347-3362.
[4]. Yoshino J, Mills KF, Yoon MJ, Imai S. Nicotinamide mononucleotide, a key NAD(+) intermediate, treats the pathophysiology of diet- and age-induced diabetes in mice. Cell Metab. 2011 Oct 5;14(4):528-36.
[5]. Mills KF, Yoshida S, Stein LR, Grozio A, Kubota S, Sasaki Y, Redpath P, Migaud ME, Apte RS, Uchida K, Yoshino J, Imai SI. Long-Term Administration of Nicotinamide Mononucleotide Mitigates Age-Associated Physiological Decline in Mice. Cell Metab. 2016 Dec 13;24(6):795-806.
| 細胞実験[1]: | |
細胞株 | 複製老化モデル |
準備方法 | 老化線維芽細胞の集団(p36.5)と、より前の2つの継代集団 (p19とp26)を5mMのNAMで3日間処理した。 |
反応条件 | 5mMのニコチンアミド；72時間 |
アプリケーション | NAMの処理は、ROSレベルを減らし、老化線維芽細胞における老化表現型の出現を減少させる。 |
| 動物実験 [2]: | |
動物モデル | SDとHFDを投与したマウスモデル |
準備方法 | SDあるいはHFDを投与されたマウスには、一生を渡ってニコチンアミド(0.5と1.0g/kg)を補給した。 |
投与形態 | ニコチンアミド(0.5/1.0 mg/kg)/日；経口投与 |
アプリケーション | ニコチンアミド(0.5/1.0 mg/kg)の処理は、最大の寿命を影響せずに、全身の生理機能と生体内の代謝を変えられる。 |
References: | |
| Cas No. | 98-92-0 | SDF | |
| 同義語 | Niacinamide, Nicotinic Acid Amide, NSC 13128, NSC 27452, Vitamin B3 Amide | ||
| Chemical Name | pyridine-3-carboxamide | ||
| Canonical SMILES | C1=CC(=CN=C1)C(=O)N | ||
| Formula | C6H6N2O | M.Wt | 122.13 |
| 溶解度 | DMF: 15 mg/ml,DMSO: 15 mg/ml,Ethanol: 2.5 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 8.188 mL | 40.94 mL | 81.88 mL |
| 5 mM | 1.6376 mL | 8.188 mL | 16.376 mL |
| 10 mM | 818.8 μL | 4.094 mL | 8.188 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)