Nicotinamide (Vitamin B3) (Synonyms: Niacinamide, Nicotinic Acid Amide, NSC 13128, NSC 27452, Vitamin B3 Amide) |
| Catalog No.GN10347 |
Nicotinamide (Vitamin B3) is an amide derivative of vitamin B3 that can inhibit the activity of SIRT1 (IC50: 50-180 μM) and SIRT2 (IC50: 2 μM). Nicotinamide can also increase the levels of NAD+, ATP, and ROS in cells .
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Cas No.: 98-92-0
Sample solution is provided at 25 µL, 10mM.
Nicotinamide (Vitamin B3) is an amide derivative of vitamin B3 that can inhibit the activity of SIRT1 (IC50: 50-180 μM) and SIRT2 (IC50: 2 μM). Nicotinamide can also increase the levels of NAD+, ATP, and ROS in cells [3].
Nicotinamide (5mM ,72h) treat senescent fibroblasts cells reduces ROS levels and the appearance of senescence phenotypes in senescent fibroblasts[1]. Nicotinamide (50-200μM) treat HepG2 cells enhanced NAD+ biosynthesis in a dose-dependent manner[4] .
Nicotinamide (0.5/1.0 mg/kg) Treatment Alters Whole-Body Physiology and In Vivo Metabolism without Affecting Maximum Lifespan[2]. Long-Term Nicotinamide（300 mg/kg/day；between 6 and 12 months） Administration Reverses Age-Associated Gene Expression Changes in Peripheral Tissues and Enhances Mitochondrial Respiratory Capacity in Skeletal Muscle[5].
References:
[1]. Kwak JY, Ham HJ, Kim CM, Hwang ES. Nicotinamide exerts antioxidative effects on senescent cells. Mol Cells. 2015 Mar;38(3):229-35.
[2]. Mitchell SJ, Bernier M, Aon MA, Cortassa S, Kim EY, Fang EF, Palacios HH, Ali A, Navas-Enamorado I, Di Francesco A, Kaiser TA, Waltz TB, Zhang N, Ellis JL, Elliott PJ, Frederick DW, Bohr VA, Schmidt MS, Brenner C, Sinclair DA, Sauve AA, Baur JA, de Cabo R. Nicotinamide Improves Aspects of Healthspan, but Not Lifespan, in Mice. Cell Metab. 2018 Mar 6;27(3):667-676.e4.
[3].Hwang ES, Song SB. Nicotinamide is an inhibitor of SIRT1 in vitro, but can be a stimulator in cells. Cell Mol Life Sci. 2017 Sep;74(18):3347-3362.
[4]. Yoshino J, Mills KF, Yoon MJ, Imai S. Nicotinamide mononucleotide, a key NAD(+) intermediate, treats the pathophysiology of diet- and age-induced diabetes in mice. Cell Metab. 2011 Oct 5;14(4):528-36.
[5]. Mills KF, Yoshida S, Stein LR, Grozio A, Kubota S, Sasaki Y, Redpath P, Migaud ME, Apte RS, Uchida K, Yoshino J, Imai SI. Long-Term Administration of Nicotinamide Mononucleotide Mitigates Age-Associated Physiological Decline in Mice. Cell Metab. 2016 Dec 13;24(6):795-806.
| Cell experiment [1]: | |
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Cell lines |
Replicative senescence model |
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Preparation Method |
A population of senescent fibroblasts (p36.5), along with two earlier passage populations (p19 and p26), was treated with 5 mM NAM for 3 days. |
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Reaction Conditions |
Nicotinamide:5mM ;72h. |
|
Applications |
NAM treatment reduces ROS levels and the appearance of senescence phenotypes in senescent fibroblasts. |
| Animal experiment [2]: | |
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Animal models |
SD and HFD-fed mouse models |
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Preparation Method |
Mice with SD or HFD supplemented with Nicotinamide (0.5 and 1.0 g/kg ) for the remainder of their lives. |
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Dosage form |
Nicotinamide (0.5/1.0 mg/kg)/ day;po |
|
Applications |
Nicotinamide (NAM) Treatment Alters Whole-Body Physiology and In Vivo Metabolism without Affecting Maximum Lifespan. |
|
References: |
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| Cas No. | 98-92-0 | SDF | |
| Synonyms | Niacinamide, Nicotinic Acid Amide, NSC 13128, NSC 27452, Vitamin B3 Amide | ||
| Chemical Name | pyridine-3-carboxamide | ||
| Canonical SMILES | C1=CC(=CN=C1)C(=O)N | ||
| Formula | C6H6N2O | M.Wt | 122.13 |
| Solubility | DMF: 15 mg/ml,DMSO: 15 mg/ml,Ethanol: 2.5 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 8.188 mL | 40.94 mL | 81.88 mL |
| 5 mM | 1.6376 mL | 8.188 mL | 16.376 mL |
| 10 mM | 0.8188 mL | 4.094 mL | 8.188 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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