Polyinosinic-polycytidylic acid sodium |
カタログ番号GC60297
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Polyinosinic-polycytidylic acid sodium（Poly I：C）はウイルス感染を模倣し、Toll-like受容体３（TLR3）やレチノ酸摂動生酸性酸などの特定のパターンの認識受容体（PRR）をトリガーすることにより遺伝子I（RIG-I）受容体に宿主免疫応答を引き出すことができる合成DSRNAである。RIG-Iおよびメラノーマ分化関連を含まれている。
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Cas No.: 42424-50-0
Sample solution is provided at 25 µL, 10mM.
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Polyinosinic-polycytidylic acid sodium（Poly I：C）はウイルス感染を模倣し、Toll-like受容体３（TLR3）やレチノ酸摂動生酸性酸などの特定のパターンの認識受容体（PRR）をトリガーすることにより遺伝子I（RIG-I）受容体に宿主免疫応答を引き出すことができる合成DSRNAである。RIG-Iおよびメラノーマ分化関連を含まれている。Poly I：Cはがん免疫療法のためのワクチン補助剤として使える[1-3]。
poly（i：c）（0.2 mLの細胞懸濁液で20 µgの量で存在している）電気駆動AML細胞はDCとNKの細胞機能を有効に活性化させ、腫瘍細胞の殺害に関してNK-DCクロストークを刺激する[4]。过Poly I：C（100 µg/ml; 72 h）でがん細胞におけるTLR3の伝達経路を活性化させ、HeLa細胞（子宮頸がん）がタンパク質合成阻害剤シクロヘキシミドによって誘導されるアポトーシスに依存性が高くなる[5]。
Poly I：C(10 µg/g;p.o.)の暴露は重度の腸損傷と炎症を引き起こす[6]。ウイルスTLR3アゴニスト（I：C）（20/200 µg）先天性免疫反応を調製でき、大腸菌K1髄膜脳炎に対する好中球減少性鼠の耐性を強化する[7]。poly(I:C)（25 µg; i.p;第９日目と第２１日目から6回始める）+R848は肺がんおよび繊維肉腫免疫能力のある鼠モデルの腫瘍の成長と転移を大幅に予防できる[8]。
References:
[1]. Zhao X, Ai M, et,al. Poly I:C-induced tumor cell apoptosis mediated by pattern-recognition receptors. Cancer Biother Radiopharm. 2012 Nov;27(9):530-4. doi: 10.1089/cbr.2012.1226. Epub 2012 Oct 12. PMID: 23062195.
[2]. Zhao X, Ai M, et,al.Poly I:C-induced tumor cell apoptosis mediated by pattern-recognition receptors. Cancer Biother Radiopharm. 2012 Nov;27(9):530-4. doi: 10.1089/cbr.2012.1226. Epub 2012 Oct 12. PMID: 23062195.
[3]. Kato H, Takeuchi O, et,al.Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature. 2006 May 4;441(7089):101-5. doi: 10.1038/nature04734. Epub 2006 Apr 9. PMID: 16625202.
[4]. Lion E, Anguille S, et,al.Poly(I:C) enhances the susceptibility of leukemic cells to NK cell cytotoxicity and phagocytosis by DC. PLoS One. 2011;6(6):e20952. doi: 10.1371/journal.pone.0020952. Epub 2011 Jun 17. PMID: 21698118; PMCID: PMC3117863.
[5]. Jiang Q, Wei H, et,al.Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway. BMC Cancer. 2008 Jan 17;8:12. doi: 10.1186/1471-2407-8-12. PMID: 18199340; PMCID: PMC2242792.
[6]. Ginzel M, Yu Y, et,al.The viral dsRNA analogue poly (I:C) induces necrotizing enterocolitis in neonatal mice. Pediatr Res. 2016 Apr;79(4):596-602. doi: 10.1038/pr.2015.261. Epub 2015 Dec 17. PMID: 26679153.
[7]. Ribes S, Arcilla C, et,al.Pre-treatment with the viral Toll-like receptor 3 agonist poly(I:C) modulates innate immunity and protects neutropenic mice infected intracerebrally with Escherichia coli. J Neuroinflammation. 2020 Jan 17;17(1):24. doi: 10.1186/s12974-020-1700-4. PMID: 31952519; PMCID: PMC6969464.
[8]. Anfray C, Mainini F, et,al.Intratumoral combination therapy with poly(I:C) and resiquimod synergistically triggers tumor-associated macrophages for effective systemic antitumoral immunity. J Immunother Cancer. 2021 Sep;9(9):e002408. doi: 10.1136/jitc-2021-002408. PMID: 34531246; PMCID: PMC8449972.
| 細胞実験[1]: | |
細胞株 | HeLa細胞 |
準備方法 | 単独でPoly I：C（100 µg/mL），CHX（1.5 µg/ml）またはPoly I：C Plus CHXで腫瘍細胞を培養する。 |
反応条件 | 100 µg/ml; 72 h |
アプリケーション | Poly I：Cの処理は腫瘍細胞はCHXが誘発した細胞死により敏感になる。 |
| 動物実験 [2]: | |
動物モデル | 四歳のC57BL/6J幼年期の鼠 |
準備方法 | 鼠には毎日二回窒息と低体温症がストレスを受ける。鼠には式のみ（FO）、LPSまたはPoly I：Cを含んでいるフォーミュラで供給される。72時間後、鼠を安楽死処分する。 |
投与形態 | 10 µg/g; p.o. |
アプリケーション | Poly I：C(10 µg/g;p.o.)の暴露は重度の腸損傷と炎症を引き起こす。 |
References: | |
| Cas No. | 42424-50-0 | SDF | |
| Chemical Name | 5'-Inosinic acid, homopolymer, complex with 5'-cytidylic acid homopolymer, sodium salt (1:1:x) | ||
| Canonical SMILES | [Polyinosinic-polycytidylic acid (sodium)] | ||
| Formula | (C10H13N4O8P)x.(C9H14N3O8P)x.xNa | M.Wt | 695.403 |
| 溶解度 | Water : 10 mg/mL (Need ultrasonic and warming) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.438 mL | 7.1901 mL | 14.3802 mL |
| 5 mM | 287.6 μL | 1.438 mL | 2.876 mL |
| 10 mM | 143.8 μL | 719 μL | 1.438 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)