Protectin D1-d5 (Synonyms: Neuroprotectin D1-d5, NPD1-d5, PD1-d5) |
カタログ番号GC48993
One-Click Copy Product Info
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プロテクチンD1の定量化のための内部標準
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: N/A
Sample solution is provided at 25 µL, 10mM.
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Protectin D1-d5 is intended for use as an internal standard for the quantification of protectin D1 by GC- or LC-MS. Protectin D1 is a specialized pro-resolving mediator (SPM) synthesized from docosahexaenoic acid .1 DHA is oxidized to 16S,17S-epoxy protectin, which is converted to protectin D1 enzymatically. Protectin D1 increases phagocytosis of apoptotic polymorphonuclear leukocytes (PMNs) by macrophages in a non-phlogistic manner and is generated in vitro during macrophage-apoptotic interactions.2 It enhances phagocytosis in mice after 24 hours, but not at the initiation or peak of inflammation. It also decreases PMN infiltration in a zymosan-induced mouse model of inflammation when administered at a dose of 300 ng per animal. Protectin D1 (200 µg, i.v.) inhibits increases in neutrophil counts in bronchoalveolar fluid (BALF) and lung myeloperoxidase activity in a mouse model of pulmonary injury and inflammation induced by intratracheal LPS instillation.3 It also decreases pulmonary edema and promotes neutrophil apoptosis in BALF.
1.Rodriguez, A.R., and Spur, B.W.Total synthesis of pro-resolving and tissue-regenerative protectin sulfido-conjugatesTetrahedron Lett.56(42)5811-5815(2015) 2.Schwab, J.M., Chiang, N., Arita, M., et al.Resolvin E1 and protectin D1 activate inflammation-resolution programmesNature447(7146)869-874(2007) 3.Li, X., Li, C., Liang, W., et al.Protectin D1 promotes resolution of inflammation in a murine model of lipopolysaccharide-induced acute lung injury via enhancing neutrophil apoptosisChin. Med. J. (Engl.)127(5)810-814(2014)
| Cas No. | N/A | SDF | |
| 同義語 | Neuroprotectin D1-d5, NPD1-d5, PD1-d5 | ||
| Canonical SMILES | O[C@@H](C/C=C\C([2H])([2H])C([2H])([2H])[2H])/C=C\C=C\C=C\[C@H](O)C/C=C\C/C=C\CCC(O)=O | ||
| Formula | C22H27D5O4 | M.Wt | 365.5 |
| 溶解度 | Ethanol: 1 mg/ml | Storage | -80°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.736 mL | 13.6799 mL | 27.3598 mL |
| 5 mM | 547.2 μL | 2.736 mL | 5.472 mL |
| 10 mM | 273.6 μL | 1.368 mL | 2.736 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solution in ethanol
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)