YSK05 |
カタログ番号GC52084
One-Click Copy Product Info
|
YSK05 は、pH に敏感なカチオン性脂質です。 YSK05 は、非ウイルスベクターの細胞内輸送を改善します。 YSK05-MEND は、非常に優れた遺伝子サイレンシング活性と溶血活性を示します。 YSK05 は、PEG 化によるエンドソーム エスケープの抑制を克服します。 YSK05 は、in vitro および in vivo の両方で siRNA 送達を効果的に強化します。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1318793-78-0
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
YSK05 is an ionizable amino lipid (apparent pKa = ~6.5) that has been used in combination with other lipids in the formation of liposomes and lipid nanoparticles (LNPs).1,2,3 Administration of cyclic di-GMP in YSK05-containing liposomes increases serum levels of IFN-β, IFN-γ, IL-6, and TNF-α and activates natural killer (NK) cells in mice, as well as decreases lung metastasis in a B16/F10 murine melanoma model of metastasis.2 Intravenous administration of factor VII siRNA in YSK05-containing LNPs reduces factor VII activity in isolated mouse plasma.3
1.Sato, Y., Hatakeyama, H., Sakurai, Y., et al.A pH-sensitive cationic lipid facilitates the delivery of liposomal siRNA and gene silencing activity in vitro and in vivoJ. Control. Release163(3)267-276(2012) 2.Nakamura, T., Miyabe, H., Hyodo, M., et al.Liposomes loaded with a STING pathway ligand, cyclic di-GMP, enhance cancer immunotherapy against metastatic melanomaJ. Control. Release216149-157(2015) 3.Sato, Y., Note, Y., Maeki, M., et al.Elucidation of the physicochemical properties and potency of siRNA-loaded small-sized lipid nanoparticles for siRNA deliveryJ. Control. Release22948-57(2016)
| Cas No. | 1318793-78-0 | SDF | |
| Canonical SMILES | CCCCC/C=C\C/C=C\CCCCCCCCOC1(CCN(CC1)C)OCCCCCCCC/C=C\C/C=C\CCCCC | ||
| Formula | C42H77NO2 | M.Wt | 628.1 |
| 溶解度 | Ethanol: 25 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.5921 mL | 7.9605 mL | 15.921 mL |
| 5 mM | 318.4 μL | 1.5921 mL | 3.1842 mL |
| 10 mM | 159.2 μL | 796.1 μL | 1.5921 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >90.00% Appearance: A solution in ethanol
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)