Apelin-12 (human, mouse, rat, bovine) (acetate) (Synonyms: RPRLSHKGPMPF) |
| Catalog No.GC52427 |
An endogenous agonist of the APJ receptor
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Apelin-12 is a 12-amino acid peptide fragment corresponding to residues 66-77 of apelin and an endogenous agonist of the apelin receptor (APJ), with roles in viral immunity, digestion, cell cycle regulation, and vasodilation.1 It induces calcium mobilization in APJ-Gαq16 cells expressing human APJ (EC50 = 0.18 nM). Apelin-12 inhibits HIV-1 and HIV-2 infection in NP-2/CD4/APJ cells (mean IC50 = 63 µg/ml).2 It inhibits or stimulates the expression of genes associated with apoptosis, mitosis, and DNA repair in IEC-6 and Caco-2 cells, respectively, when used at a concentration of 0.01 µM.3 Apelin-12 (100-1,000 nM) induces the production of nitric oxide (NO) via PI3K and Akt signaling pathways in human umbilical vein endothelial cells (HUVECs).4 In vivo, apelin-12 (20-100 µg/kg) increases gastric acid secretion, as well as stomach histamine synthesis and secretion, in rats.5 It also reduces mean arterial pressure (MAP) in rats when administered at a dose of 15 µg/kg.6
1.Giddings, A., Runyon, S., Thomas, J., et al.Development of a functional HTS assay for the APJ receptorInt. J. High Throughput Screen.139-47(2010) 2.Zou, M.X., Liu, H.Y., Haraguchi, Y., et al.Apelin peptides block the entry of human immunodeficiency virus (HIV)FEBS Lett.473(1)15-18(2000) 3.Antushevich, H., Krawczynska, A., Kapica, M., et al.Effect of apelin on mitosis, apoptosis and DNA repair enzyme OGG 1/2 expression in intestinal cell lines IEC-6 and Caco-2Folia Histochem. Cytobiol.52(1)51-59(2014) 4.Busch, R., Strohbach, A., Pennewitz, M., et al.Regulation of the endothelial apeliPJ system by hemodynamic fluid flowCell. Signal.27(7)1286-1296(2015) 5.Ohno, S., Yakabi, K., Ro, S., et al.Apelin-12 stimulates acid secretion through an increase of histamine release in rat stomachsRegul. Pept.174(1-3)71-79(2012) 6.Lee, D.K., Saldivia, V.R., Nguyen, T., et al.Modification of the terminal residue of apelin-13 antagonizes its hypotensive actionEndocrinology146(1)231-236(2005)
| Cas No. | SDF | ||
| Synonyms | RPRLSHKGPMPF | ||
| Canonical SMILES | O=C([C@H]1N(CCC1)C(CNC([C@H](CCCCN)NC([C@@H](NC([C@H](CO)NC([C@H](CC(C)C)NC([C@H](CCCNC(N)=N)NC([C@H]2N(CCC2)C([C@@H](N)CCCNC(N)=N)=O)=O)=O)=O)=O)CC3=CN=CN3)=O)=O)=O)N[C@@H](CCSC)C(N4[C@@H](CCC4)C(N[C@H](C(O)=O)CC5=CC=CC=C5)=O)=O.CC(O)=O | ||
| Formula | C64H103N21O14S ? XC2H4O2 | M.Wt | 1422.7 |
| Solubility | DMF: insol,DMSO: insol,Ethanol: insol,PBS (pH 7.2): 5 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.7029 mL | 3.5144 mL | 7.0289 mL |
| 5 mM | 0.1406 mL | 0.7029 mL | 1.4058 mL |
| 10 mM | 0.0703 mL | 0.3514 mL | 0.7029 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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