Poly(I:C) (Synonyms: Polyinosinic-polycytidylic acid)

Name: Poly(I:C)
Brand: GlpBio
SKU: GC14710
Availability: InStock
Rating: 5 (28 reviews)

Catalog No.GC14710

Poly(I:C)는 synthetic double-stranded RNA(dsRNA) 유사물질로서, 가장 강력한 인터페이런(IFN) 유도체로 작용하는 면역 자극제이다.

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Poly(I:C) Chemical Structure

Cas No.: 24939-03-5

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10mM (in 1mL Water)	US$38.00	재고 있음
10mg	US$70.00	재고 있음
50mg	US$220.00	재고 있음

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Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

Product has been cited by 2 publications

Description Protocol Chemical Properties Quality Control Product Documents

Related Biological Data

The DDX58 and downstream signalling pathway was activated by Dox-induced endogenous double-stranded RNAs.(B) Immunofluorescence staining of dsRNA in MDA-MB-231cells with anti-dsRNA J2 antibody (green) and DAPI (blue). Cells transfected with poly I:C were included as a positive control for dsRNAs.
First, we checked the endogenous expression level of dsRNAs in MDA-MB-231 cells following 6 hrs Dox treatment. Poly(I:C) (10 mg/ml) (GlpBio) was used as positive control.
Frontiers in Oncology 14 (2024): 1356778. PMID: 38549944 IF: 4.7003

Product Documents

Quality Control & SDS

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Purity: >90.00%

Protocol

Cell experiment [1]:
Cell lines	BEAS-2B cells
Preparation Method	BEAS-2B cells were seeded in 6-well plates one day before Poly (I:C) treatment. One hour before infection at multiplicity of infection (MOI) 0.01, 1, or 5, the cells were pre-treated with 4 µg/ml of Poly (I:C) or left untreated. After adsorption, the cells were maintained in the medium with or without Poly (I: C) (4 µg/ml). The CPE was observed at 24, 48, and 72 h postinfection (p.i.) under a microscope.
Reaction Conditions	4 µg/ml Poly (I: C) for 24/48/72 h
Applications	Poly (I:C) suppressed cytopathic effects (CPE) induced by CHIKV infection in BEAS-2B cells in the presence of Poly (I:C) and inhibited the replication of CHIKV in the cells. The virus titers of Poly (I:C)-treated cells were much lower compared with those of untreated cells. CHIKV infection and Poly (I:C) treatment of BEAS-2B cells induced the production of IFN-β and increased the expression of anti-viral genes, including IFN-α, IFN-β, MxA, and OAS. Both Poly (I:C) and CHIKV infection upregulate the expression of TLR3 in BEAS-2B cells.
Animal experiment [2]:
Animal models	C57BL/6J mouse
Preparation Method	Injection of MC38 cells into C57BL/6J mice and the subsequent intraperitoneal injections of Poly(I:C),Mice were treated with 50 µg Poly(I:C) by intraperitoneal injection on days 8, 11, 14, 17, 20 and 23.
Dosage form	50 µg Poly(I:C) by intraperitoneal injection on days 8, 11, 14, 17, 20 and 23.
Applications	Poly(I:C)-activated macrophages displayed enhanced phagocytosis after CD47 blockade. Compared with mice receiving monotherapy, those received Poly(I:C) in combination with anti-CD47 mAb exhibited significantly inhibited tumor growth. Moreover, in vivo delivery of Poly(I:C) plus anti-CD47 mAb to tumor-bearing mice altered the tumor immune microenvironment.
References: [1]: Li YG, Siripanyaphinyo U, et,al. Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells. Virol J. 2012 Jun 14;9:114. doi: 10.1186/1743-422X-9-114. PMID: 22698190; PMCID: PMC3490739. [2]: Zhong C, Wang L, et,al. Poly(I:C) enhances the efficacy of phagocytosis checkpoint blockade immunotherapy by inducing IL-6 production. J Leukoc Biol. 2021 Dec;110(6):1197-1208. doi: 10.1002/JLB.5MA0421-013R. Epub 2021 May 14. PMID: 33988261.

Poly(I:C)는 synthetic double-stranded RNA(dsRNA) 유사물질로서, 가장 강력한 인터페이런(IFN) 유도체로 작용하는 면역 자극제이다.Toll-like receptor-3（TLR3）는 활성화제^[1]이다.

Poly (I:C)는 BEAS-2B 세포에서 CHIKV 감염으로 인한 세포병변효과(CPE)를 억제하고 세포 내 CHIKV의 복제를 억제했다. Poly (I:C)와 CHIKV 감염은 BEAS-2B 세포에서 TLR3의 표현을 상승시켰다^[1]. DsRNA poly(I:C) up-regulated는 공경 암 세포에서 IFNβ와 세포 사멸과 관련된 유전자의 표현을 상승시켜 내재적과 외재적 사멸 경로를 활성화하고 결국 세포 사멸을 유도했다^[8]. 덴드리트 셀의 안정적인 성숙은 polyriboinosinic polyribocytidylic acid (Poly (I:C))의 추가를 통해 간단하게 유도될 수 있다^[3]. Poly (I:C)는 DNA 손상과 첫 번째 분열 시의 이상한 방추 형태를 통해 체외 실험 수정 성공률을 감소시켰고, 면역 반응을 유도하고 블라스토시스트 세포의 세포사멸을 촉진하여 조기 배아 발육을 억제했다^[9]. TLR7/8 활성제 R848이나 CL075를 포함한 Poly (I:C)와 혼합한 혼합물은 3일 성숙한 덴드리트 세포를 산생시켰다^[2].

Poly (I:C)가 활성화한 거대세포는 CD47 차단 후에 증강된 팽창작용을 보였다. 단일 치료를 받은 쥐와 비교하여, Poly (I:C)와 항CD47 mAb를 조합하여 치료받은 쥐의 종양 성장이 현저히 억제되었다^[4]. 체내 실험에서 Poly (I:C)는 쥐의 중성세포 표면 Mac-1의 표현을 강화하고 그들이 폐 조직으로 침투하도록 촉진했다. Poly (I:C)는 또한 쥐 조직에서의 thrombomodulin 표현을 다운 조절하고 그들의 혈장에서의 순환 가능한 수준을 낮추었다^[5]. 1.25 및 5 mg/kg Poly (I:C) 전처리가 심근경색 면적과 심장 기능 장애를 현저히 줄였다. 또한 Poly (I:C)는 자기포식 유동을 회복하고 그를 적절한 수준으로 조절함으로써 세포 생존을 현저히 촉진했다. 추가적인 클로로퀸 ^[6]을 투여한 후 p62분해와 함께 자가파지 단백질인 Beclin1과 LC3II를 증가시킴으로써 세포 생존을 현저히 촉진하였다. 쥐에서, Poly (I:C) 노출 후 DA-D2 수용체 결합의 현저한 감소, 뇌수경 부피 감소 및 MOG 면역 표시로 인한 뇌백색 질 손상, 그리고 운동 활동, 신경근육 강도 및 운동 조정 능력의 현저한 감소는 초기 인생 바이러스 감염의 운동 장애 및 운동 감소의 영향을 나타낸다^[7].

References:
[1]: Li YG, Siripanyaphinyo U, et,al. Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells. Virol J. 2012 Jun 14;9:114. doi: 10.1186/1743-422X-9-114. PMID: 22698190; PMCID: PMC3490739.
[2]: Spranger S, Javorovic M, et,al. Generation of Th1-polarizing dendritic cells using the TLR7/8 agonist CL075. J Immunol. 2010 Jul 1;185(1):738-47. doi: 10.4049/jimmunol.1000060. Epub 2010 May 28. PMID: 20511554.
[3]: Verdijk RM, Mutis T, et,al. Polyriboinosinic polyribocytidylic acid (poly(I:C)) induces stable maturation of functionally active human dendritic cells. J Immunol. 1999 Jul 1;163(1):57-61. PMID: 10384099.
[4]: Zhong C, Wang L, et,al. Poly(I:C) enhances the efficacy of phagocytosis checkpoint blockade immunotherapy by inducing IL-6 production. J Leukoc Biol. 2021 Dec;110(6):1197-1208. doi: 10.1002/JLB.5MA0421-013R. Epub 2021 May 14. PMID: 33988261.
[5]: Cai X, Panicker SR, et,al.Protective Role of Activated Protein C against Viral Mimetic Poly(I:C)-Induced Inflammation. Thromb Haemost. 2021 Nov;121(11):1448-1463. doi: 10.1055/s-0041-1726093. Epub 2021 Mar 11. PMID: 33706396; PMCID: PMC8433266.
[6]: Chen E, Chang H, et,al. Poly(I:C) attenuates myocardial ischemia/reperfusion injury by restoring autophagic function. FASEB J. 2022 May;36(5):e22317. doi: 10.1096/fj.202101220RR. PMID: 35438806.
[7]:Singh B, Dhuriya YK, et,al. Early life exposure to poly I:C impairs striatal DA-D2 receptor binding, myelination and associated behavioural abilities in rats. J Chem Neuroanat. 2021 Dec;118:102035. doi: 10.1016/j.jchemneu.2021.102035. Epub 2021 Sep 28. PMID: 34597812.
[8]: Meng X, Cui X, et,al. poly(I:C) synergizes with proteasome inhibitors to induce apoptosis in cervical cancer cells. Transl Oncol. 2022 Apr;18:101362. doi: 10.1016/j.tranon.2022.101362. Epub 2022 Feb 9. PMID: 35151092; PMCID: PMC8842080.
[9]: Wang Z, Chen S, et,al. Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development. J Reprod Immunol. 2022 Jun;151:103635. doi: 10.1016/j.jri.2022.103635. Epub 2022 Apr 30. PMID: 35525084.

Cas No.	24939-03-5	SDF
Synonyms	Polyinosinic-polycytidylic acid
Chemical Name	((2R,3S,4R,5R)-3,4-dihydroxy-5-(6-oxo-3H-purin-9(6H)-yl)tetrahydrofuran-2-yl)methyl dihydrogen phosphate compound with ((2R,3S,4S,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate (1:1)
Canonical SMILES	O[C@@H]([C@H](N1C(NC=NC2=O)=C2N=C1)O[C@@H]3COP(O)(O)=O)[C@@H]3O.O[C@H]([C@@H](COP(O)(O)=O)O[C@H]4N5C=CC(N)=NC5=O)[C@@H]4O
Formula	C₁₉H₂₇N₇O₁₆P₂	M.Wt	671.41
Solubility	≥ 21.5mg/mL in Water	Storage	Store at -20°C
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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		1 mg	5 mg	10 mg
	1 mM	1.4894 mL	7.447 mL	14.894 mL
	5 mM	0.2979 mL	1.4894 mL	2.9788 mL
	10 mM	0.1489 mL	0.7447 mL	1.4894 mL

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Average Rating: 5 ★★★★★ (Based on Reviews and 28 reference(s) in Google Scholar.)

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Poly(I:C) (Synonyms: Polyinosinic-polycytidylic acid)

고객 리뷰

GlpBio Citations

Bioactive Compounds Premium Provider

Product has been cited by 2 publications

Related Biological Data

Product Documents

Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

Molarity Calculator

Dilution Calculator

In vivo Formulation Calculator (Clear solution)

리뷰

Poly(I:C) (Synonyms: Polyinosinic-polycytidylic acid)

고객 리뷰

GlpBio Citations

Bioactive Compounds Premium Provider

Product has been cited by 2 publications

Related Biological Data

Product Documents

Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

Molarity Calculator

Dilution Calculator

In vivo Formulation Calculator (Clear solution)

Related Products

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