D-Luciferin (sodium salt) |
Catalog No.GC43497 |
D-루시페린(D-(-)-루시페린) 나트륨은 생물발광 곤충에서 빛 생성을 촉매하는 루시페라제의 기질입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 103404-75-7
Sample solution is provided at 25 µL, 10mM.
D-luciferin is the natural substrate of firefly luciferase. In the presence of magnesium ions, luciferase catalyzes the reaction of luciferin with ATP, which is then oxidized to form a dioxetane structure that emits yellow-green light [1]. When the substrate luciferin is in excess, the 560 nm chemiluminescence generated by the Luciferin-luciferase luminescent reaction reaches its peak within a few seconds, and the light output is proportional to the luciferase concentration. Chemiluminescent techniques are virtually background-free, making the luciferase reporter an ideal tool for detecting low-level gene expression. 0.02 pg of luciferase can be reliably measured in a standard fluorescence counter. D-luciferin is a commonly used reporter gene for ATP detection, cell viability assay, reporter gene detection, active molecular screening and bacterial counting. D-luciferin is widely used in live animal imaging. Cells expressing the luciferase gene were transplanted into research animals and injected with D-luciferin to be able to detect changes in brightness by bioluminescence imaging (BLI)[2].
D-Luciferin has three product forms, D-Luciferin (D-Luciferin, free acid), D-Luciferin potassium salt (D-Luciferin, potassium salt) and D-Luciferin sodium salt (D-Luciferin, sodium salt ). The potassium and sodium salt forms of D-fluorescein are the most versatile because they are both readily soluble in water. Potassium salt is also the main form used in live animal testing.
References:
[1]. Giuseppe Meroni, et al. D-Luciferin, derivatives and analogues: synthesis and in vitro/in vivo luciferase-catalyzed bioluminescent activity. ARKIVOC 2009 (i) 265-288.
[2]. Sangyub Kim, et al. Optimizing live-animal bioluminescence imaging prediction of tumor burden in human prostate cancer xenograft models in SCID-NSG mice.2019 Jun;79(9):949-960. doi: 10.1002/pros.23802. Epub 2019 Apr 8.
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