Z-Lys-SBzl . HCl (Synonyms: α-N-Carbobenzoxy-L-lysine thiobenzyl ester monohydrochloride) |
Catalog No.GA23877
One-Click Copy Product Info
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Z-Lys-SBzl . HCl is a lysine derivative and a protease assay substrate.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 69861-89-8
Sample solution is provided at 25 µL, 10mM.
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Z-Lys-SBzl . HCl is a lysine derivative and a protease assay substrate. Z-Lys-SBzl . HCl serves as a thioester substrate for photometric assays to detect enzyme activity. Z-Lys-SBzl . HCl can be used in research related to protease activity determination and as an intermediate in peptide synthesis[1-4].
References:
[1] Gasparian ME, Ostapchenko VG, Schulga AA, et al. Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli. Protein Expr Purif. 2003 Sep;31(1):133-9.
[2] Gasparian ME, Ostapchenko VG, Dolgikh DA, et al. Biochemical characterization of human enteropeptidase light chain. Biochemistry (Mosc). 2006 Feb;71(2):113-9.
[3] Lawson JH, Mann KG. Cooperative activation of human factor IX by the human extrinsic pathway of blood coagulation. J Biol Chem. 1991 Jun 15;266(17):11317-27.
[4] Wilharm E, Tschopp J, Jenne DE. Biological activities of granzyme K are conserved in the mouse and account for residual Z-Lys-SBzl activity in granzyme A-deficient mice. FEBS Lett. 1999 Oct 1;459(1):139-42.
Measurement of Esterolytic Activity of Complement Factor D Using Z-Lys-SBzl . HCl[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent Preparation: Dissolve Z-Lys-SBzl . HCl in the reaction buffer for later use. Prepare a 0.10M Hepes buffer containing 0.50M NaCl and 9.8% DMSO, and a solution of 4,4'-dithiodipyridine.
2. Reaction System Setup: In a cuvette, establish a final reaction system with a volume of 1mL. The system contains the aforementioned Hepes buffer, 0.19mM Z-Lys-SBzl . HCl substrate, and 1μM of purified human complement Factor D.
3. Enzyme Activity Detection: Place the cuvette in a spectrophotometer and monitor the absorbance change at 324nm under 27°C conditions. The benzyl mercaptan released by Factor D hydrolysis of Z-Lys-SBzl·HCl immediately reacts with 4,4’-dithiodipyridine in the system to generate 4-thiopyridone. The molar extinction coefficient (ε) of this product at 324nm is 19,800M⁻¹ cm⁻¹. The initial rate of the enzymatic reaction (v₀) is calculated from the initial rate of increase in absorbance.
Precautions:
(1) To verify the completion of the reaction, an excess amount of trypsin (e.g., 5μg) can be added to the system after measuring the initial rate to completely hydrolyze the remaining substrate in a short time, allowing for precise calculation of the initial substrate concentration.
(2) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] González-Rubio C, González-Muñiz R, Jiménez-Clavero MA, et al. Factor J, an inhibitor of the classical and alternative complement pathway, does not inhibit esterolysis by factor D. Biochim Biophys Acta. 1996 Jul 18;1295(2):174-8.
| Cas No. | 69861-89-8 | SDF | |
| Synonyms | α-N-Carbobenzoxy-L-lysine thiobenzyl ester monohydrochloride | ||
| Formula | C21H26N2O3S · HCl | M.Wt | 422.98 |
| Solubility | DMSO : 250 mg/mL (591.06 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3642 mL | 11.8209 mL | 23.6418 mL |
| 5 mM | 472.8 μL | 2.3642 mL | 4.7284 mL |
| 10 mM | 236.4 μL | 1.1821 mL | 2.3642 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)