PJ34 hydrochloride (Synonyms: PJ 34 Hydrochloride) |
Catalog No.GC10145
One-Click Copy Product Info
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PJ34 hydrochloride is an effective and specific PARP inhibitor with an IC50 value of 110nM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 344458-15-7
Sample solution is provided at 25 µL, 10mM.
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PJ34 hydrochloride is an effective and specific PARP inhibitor with an IC50 value of 110nM [1]. PJ34 hydrochloride promotes cell death by inhibiting PARP1-mediated DNA repair, and leads to a decrease in NF-κB expression and specifically blocks the mitosis of cancer cells[2]. PJ34 hydrochloride has been widely used to kill tumor cells and has been employed in the development of novel combined therapies to efficiently enhance the cytotoxicity of chemotherapy drugs[3].
In vitro, PJ34 hydrochloride treatment for 48 hours significantly inhibited the viability of OVCAR-3 cells and SKOV-3 cells, with the IC50 values being 18.7µM and 28.5µM, respectively[4]. Treatment with 50µM PJ34 hydrochloride for 72 hours induced cell cycle arrest and apoptosis in HTLV-I-transformed MT-2 cells[5]. Treatment with 10µM PJ34 hydrochloride for 24 hours resulted in mitotic defects in M14 cells and promoted abnormal accumulation of actin in the nucleolus[6].
In vivo, PJ34 hydrochloride treatment via intraperitoneal injection at a dose of 10mg/kg three times weekly for 4 weeks alleviated the accumulation of triglycerides (TG) in the liver of alcohol-fed mice and reduced the hepatic gene expression of sterol regulatory element-binding protein 1c, DGAT1, and DGAT2[7]. Intraperitoneal injection of PJ34 hydrochloride (50μg) at 2 hours before and 6 hours after middle cerebral artery occlusion (MCAo) significantly reduced the cerebral infarct volume in SV/129 mice [8].
References:
[1] Iwashita A, Tojo N, Matsuura S, et al. A novel and potent poly (ADP-ribose) polymerase-1 inhibitor, FR247304 (5-chloro-2-[3-(4-phenyl-3, 6-dihydro-1 (2 H)-pyridinyl) propyl]-4 (3 H)-quinazolinone), attenuates neuronal damage in in vitro and in vivo models of cerebral ischemia[J]. The Journal of pharmacology and experimental therapeutics, 2004, 310(2): 425-436.
[2] Cohen-Armon M. The modified phenanthridine PJ34 unveils an exclusive cell-death mechanism in human cancer cells[J]. Cancers, 2020, 12(6): 1628.
[3] Mann M, Chauhan S S, Kumar S, et al. PJ34-mediated PARP-1 enzymatic activity inhibition in cervical cancer modulates the cellular response to cisplatin[J]. Cancer Research, 2016, 76(14_Supplement): 2152-2152.
[4] Güler A E, Tuncer M C, Özdemir İ. Synergistic Antitumor Activity of Curcumin and the PARP1 Inhibitor PJ34 in Platinum-Sensitive and Resistant Ovarian Cancer Cells[J]. Cancers, 2026, 18(4): 620.
[5] Bai X T, Moles R, Chaib-Mezrag H, et al. Small PARP inhibitor PJ-34 induces cell cycle arrest and apoptosis of adult T-cell leukemia cells[J]. Journal of hematology & oncology, 2015, 8(1): 117.
[6] Chevanne M, Zampieri M, Caldini R, et al. Inhibition of PARP activity by PJ‐34 leads to growth impairment and cell death associated with aberrant mitotic pattern and nucleolar actin accumulation in M14 melanoma cell line[J]. Journal of cellular physiology, 2010, 222(2): 401-410.
[7] Huang S, Zhang B, Chen Y, et al. Poly (ADP-ribose) polymerase inhibitor PJ34 attenuated hepatic triglyceride accumulation in alcoholic fatty liver disease in mice[J]. The Journal of pharmacology and experimental therapeutics, 2018, 364(3): 452-461.
[8] Abdelkarim G E, Gertz K, Harms C, et al. Protective effects of PJ34, a novel, potent inhibitor of poly (ADP-ribose) polymerase (PARP) in in vitro and in vivo models of stroke[J]. International journal of molecular medicine, 2001, 7(3): 255-260.
| Cell experiment [1]: | |
Cell lines | OVCAR-3 cells |
Preparation Method | OVCAR-3 cells were grown in RPMI-1640 medium with 10% (v/v) fetal bovine serum (FBS), antibiotic/antimycotic solution at 37°C in 5% CO2/atmosphere. Cells were seeded in 96-well plates at a density of 5×103 cells and cultured overnight. After 48h of treatment with different concentrations of PJ34 hydrochloride (0, 5, 10, 25, 50, and 100µM), the cell viability was measured. |
Reaction Conditions | 0, 5, 10, 25, 50, and 100µM; 48h |
Applications | PJ34 hydrochloride treatment significantly inhibited the cell viability of OVCAR-3 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male C57BL/6 mice |
Preparation Method | Male C57BL/6 mice (8 weeks old; 21-23g) were housed singly in a standard environment with food and water ad libitum. All mice were fed for 4 weeks with liquid diets. The pair-fed diet (PF) contained 18% protein, 35% fat, and 47% carbohydrate, whereas the alcohol-fed diet (AF) contained 18% protein, 35% fat, 11% carbohydrate, and 36% alcohol. PJ34 hydrochloride (10mg/kg) was injected intraperitoneally three times a week for 4 weeks. The same volume of physiologic saline (0.1ml/20g per day; i.p.) was injected intraperitoneally three times a week as a vehicle treatment. At the end of the experiment, liver samples were harvested for analysis. |
Dosage form | 10mg/kg; three times a week for 4 weeks; i.p. |
Applications | PJ34 hydrochloride treatment significantly reduced the accumulation of TG in the liver of alcohol-fed mice. |
References: | |
| Cas No. | 344458-15-7 | SDF | |
| Synonyms | PJ 34 Hydrochloride | ||
| Chemical Name | 2-(dimethylamino)-N-(6-oxo-5H-phenanthridin-2-yl)acetamide;hydrochloride | ||
| Canonical SMILES | CN(C)CC(=O)NC1=CC2=C(C=C1)NC(=O)C3=CC=CC=C32.Cl | ||
| Formula | C17H17N3O2.HCl | M.Wt | 331.8 |
| Solubility | ≥ 16.6 mg/mL in DMSO;Water:50 mg/mL (150.69 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0139 mL | 15.0693 mL | 30.1386 mL |
| 5 mM | 602.8 μL | 3.0139 mL | 6.0277 mL |
| 10 mM | 301.4 μL | 1.5069 mL | 3.0139 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)