Alcian Blue 8GX |
Katalog-Nr.GC67217
One-Click Copy Product Info
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Alcian Blue 8GX ist ein kationischer Kupferphthalocyanin-Farbstoff, der bekanntermaßen an Glykoproteine \u200b\u200bund Glucosaminoglykane bindet.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 33864-99-2
Sample solution is provided at 25 µL, 10mM.
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Alcian Blue 8GX is a cationic copper phthalocyanine dye well-known to bind to glycoproteins and to glucosaminoglycans[1].
References:
[1]. Candiano G, et al. Widening and diversifying the proteome capture by combinatorial peptide ligand libraries via Alcian Blue dye binding. Anal Chem. 2015;87(9):4814-4820.
This plan only provides a guide, please modify it to meet your specific needs.
1. Prepare 1-10 mg/mL Alcian Blue 8GX staining solution: add 0.6 mL glacial acetic acid to ddH2O to make the final volume 20 mL. Then add 20-200 mg of solid Alcian Blue 8GX, fully dissolve and then use a 0.22μm filter to filter and sterilize.
Note: The prepared dye solution should be stored at 4℃ away from light and is recommended to be used within one month.
2. Cell suspension staining (taking 6-well plate as an example)
(1) Centrifuge suspended cells at 1000g for 3-5 minutes. Discard the supernatant and wash twice with PBS for 5 minutes each time.
(2) Wash the adherent cells twice with PBS, add trypsin to digest the cells, and centrifuge at 1000g for 3-5 minutes after digestion is completed.
(3) Add 1 mL of dye working solution to resuspend the cells, and incubate at room temperature in the dark for 30-60 minutes. The optimal culture time for different cells is different.
(4) After the incubation, centrifuge at 1000g for 5 minutes, remove the supernatant, and add PBS to wash 2-3 times, 5 minutes each time.
(5) Resuspend the cells in serum-free cell culture medium or PBS and observe through a microscope.
3. Cell adhesion staining
(1) Culture adherent cells on sterile coverslips.
(2) Remove the coverslip from the culture medium, aspirate the excess culture medium, and place the coverslip in a humid environment.
(3) Add 100μL dye working solution from one corner of the coverslip, shake gently to evenly cover all cells with the dye, and incubate at room temperature in the dark for 30-60 minutes.
(4) Aspirate away the dye working solution and use culture solution to wash the coverslip 2 to 3 times for 5 minutes each time.
4. Microscopic examination: Use a light microscope to observe the stained cells.
Note: For your safety and health, please wear a lab coat and disposable gloves.
References:
[1]. Hiroki Ueharu,et. Isolation and Culture of Cranial Neural Crest Cells from the First Branchial Arch of Mice. 2022 Apr 5;12(7):e4371. doi: 10.21769/BioProtoc.4371.
[2]. J Tas. The Alcian blue and combined Alcian blue--Safranin O staining of glycosaminoglycans studied in a model system and in mast cells. 1977 Mar;9(2):205-30. doi: 10.1007/BF01003632.
| Cas No. | 33864-99-2 | SDF | |
| Formula | C56H68Cl4CuN16S4 | M.Wt | 1298.86 |
| Löslichkeit | DMSO : 125 mg/mL (96.24 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 769.9 μL | 3.8495 mL | 7.6991 mL |
| 5 mM | 154 μL | 769.9 μL | 1.5398 mL |
| 10 mM | 77 μL | 385 μL | 769.9 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)