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Biotin-11-UTP

Katalog-Nr.GC71769 Copy One-Click Copy Product Info

Biotin-11-UTP ist ein Biotin-markiertes Uridintriphosphat (UTP), das 11-Atome im Binder zwischen Biotin und UTP enthält.

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Biotin-11-UTP Chemische Struktur

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1 umol
765,00 $
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Sample solution is provided at 25 µL, 10mM.



Description of Biotin-11-UTP

Biotin-11-UTP is a Biotin-labeled uridine triphosphate (UTP), containing 11 atoms in the linker between biotin and UTP. Biotin-11-UTP can biotinylate transcribed RNA in vitro and has been widely used as labeling RNA probe. Biotin-11-UTP is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

References:
[1]. Rodriguez, et al. Biotin-Labeled Riboprobes to Study RNA-Binding Proteins. In: Meier, T., Fahrenholz, F. (eds) A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis. BioMethods. Birkhäuser Basel. 1996

Chemical Properties of Biotin-11-UTP

Cas No. SDF
Formula C28H40Li3N6O18P3S M.Wt 894.46
Löslichkeit Storage 4°C, sealed storage, away from moisture and light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Biotin-11-UTP

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 1.118 mL 5.59 mL 11.1799 mL
5 mM 223.6 μL 1.118 mL 2.236 mL
10 mM 111.8 μL 559 μL 1.118 mL
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In vivo Formulation Calculator (Clear solution) of Biotin-11-UTP

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

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