BODIPY 500/510 C1, C12 |
Katalog-Nr.GC26173
One-Click Copy Product Info
|
BODIPY 500/510 C1, C12 is a fluorescent fatty acid analog with maximum excitation/emission wavelengths at 500/510nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 144672-74-2
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
BODIPY 500/510 C1, C12 is a fluorescent fatty acid analog with maximum excitation/emission wavelengths at 500/510nm[1]. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability. Its fluorescence peak is relatively sharp and its quantum yield is high. It is relatively insensitive to the polarity and pH value of the environment and is relatively stable under different physiological conditions[2]. Due to its asymmetric structure, BODIPY has a variety of derivative structural products[3]. BODIPY lipid droplet dyes can easily pass through the cell membrane and enter the cell interior. They can be specifically stained by localizing on neutral lipids in the cell, so the dye can be used to label living cells and fixed cells[4, 5].
References:
[1]Varlamov O, Roberts C T, Slayden O D. Androgens Inhibit Insulin-Dependent Lipid Uptake in Visceral Fat of Female Nonhuman Primates[J]. 2010.
[2]Bumagina N A, Antina E V, Ksenofontov A A, et al. Basic structural modifications for improving the practical properties of BODIPY[J]. Coordination Chemistry Reviews, 2022, 469: 214684.
[3]Qin Y, Liu X, Jia P P, et al. BODIPY-based macrocycles[J]. Chemical Society Reviews, 2020, 49(16): 5678-5703.
[4]Jurgutis D, Jarockyte G, Poderys V, et al. Exploring BODIPY-Based sensor for Imaging of intracellular microviscosity in human breast cancer cells[J]. International Journal of Molecular Sciences, 2022, 23(10): 5687.
[5]Hsieh K, Lee Y K, Londos C, et al. Perilipin family members preferentially sequester to either triacylglycerol-specific or cholesteryl-ester-specific intracellular lipid storage droplets[J]. Journal of cell science, 2012, 125(17): 4067-4076.
This protocol is only a guide. Please modify it according to your specific needs.
1. Preparation of staining solution
(1) Prepare stock solution: Dissolve BODIPY 500/510 C1, C12 in DMSO to prepare a stock solution with a concentration of 1-10mM.
Note: Store unused stock solution at -20°C or -80°C away from light to avoid repeated freezing and thawing.
(2) Prepare working solution: Dilute the stock solution with a suitable buffer (such as serum-free medium or PBS) to prepare a working solution with a concentration of 1-10μM.
Note: Please adjust the working solution concentration according to actual conditions and prepare it before use.
2. Cell suspension staining
(1) Suspended cells: Centrifuge at 1000g for 5min at 4°C, discard the supernatant, and wash twice with PBS for 5min each time.
(2) Adherent cells: Wash twice with PBS, add trypsin to digest the cells, and centrifuge at 1000g for 3-5min after digestion.
(3) Resuspend approximately 106 cells in 1mL of BODIPY 500/510 C1, C12 working solution and incubate at 37°C in the dark for 5-30min. The optimal incubation time varies for different cells. Please explore according to the specific experimental requirements.
(4) After incubation, centrifuge at 1000g for 5min, remove the supernatant, and wash 2-3 times with PBS, each time for 5min.
(5) Resuspend the cells in pre-warmed serum-free cell culture medium or PBS and observe by fluorescence microscopy or flow cytometry.
3. Cell adhesion staining
(1) Culture adherent cells on sterile coverslips.
(2) Remove the coverslip from the culture medium, aspirate the excess culture medium, and place the coverslip in a humid environment.
(3) Add 100μL of dye working solution from one corner of the coverslip and gently shake to evenly cover all cells with the dye.
(4) Incubate at 37 °C in the dark for 5-30min. The optimal incubation time for different cells is different. Please explore it yourself according to the specific experimental needs.
(5) After the incubation, aspirate and discard the dye working solution, and wash the coverslip 2-3 times with pre-warmed culture medium.
Notes:
(1) Please adjust the concentration and incubation time of BODIPY 500/510 C1, C12 working solution according to the actual situation.
(2) It is recommended to perform a positive control in the experiment, such as incubating the control group cells with 30μM oleic acid for 8h before conducting subsequent experiments.
(3) This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, nor for food or medicine.
(4) For your safety and health, please wear a lab coat and disposable gloves when operating.
References:
[1]. Zaiguo Li, et al. Synthesis and spectral properties of cholesterol- and FTY720-containing boron dipyrromethene dyes. Synthesis and spectral properties of cholesterol- and FTY720-containing boron dipyrromethene dyes. J Org Chem. 2007 Oct 26;72(22):8376-82.
[2]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912.
| Cas No. | 144672-74-2 | SDF | |
| Canonical SMILES | [F-][B+3]1([N-]2C(C=C(C=C3)[N]1=C3C)=CC=C2CCCCCCCCCCCC(O)=O)[F-] | ||
| Formula | C22H31BF2N2O2 | M.Wt | 404.3 |
| Löslichkeit | DMSO : 100 mg/mL (247.34 mM; Need ultrasonic) | Storage | Desiccate at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4734 mL | 12.3671 mL | 24.7341 mL |
| 5 mM | 494.7 μL | 2.4734 mL | 4.9468 mL |
| 10 mM | 247.3 μL | 1.2367 mL | 2.4734 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)