WST-8 (Synonyms: Water-Soluble Tetrazolium 8) |
| Katalog-Nr.GC10543 |
WST-8 ist ein wasserlöslicher Tetrazoliumfarbstoff, der die Empfindlichkeit des auf WST-8 basierenden Assays im Vergleich zum konventionellen MTS-basierten Assay erhöht.
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Cas No.: 193149-74-5
Sample solution is provided at 25 µL, 10mM.
WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].
Reference:
[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.
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