Cholic Acid-d4 (Synonyms: Cholalic Acid-d4, Cholalin-d4)

Cholic acid-d₄ is intended for use as an internal standard for the quantification of cholic acid by GC- or LC-MS. Cholic acid is a primary bile acid.¹ It is formed from cholesterol via a multistep process catalyzed by the cytochrome P450 (CYP) isoforms CYP7A1, CYP8B1, and CYP27A1. Cholic acid is conjugated to glycine or taurine by bile acid-CoA:amino acid N-acyltransferase (BAAT) to produce glycocholic acid and taurocholic acid , respectively, in the liver, and is transformed into the secondary bile acid deoxycholic acid by intestinal microbiota.^1,2,3 It induces C. difficile colony formation in an agar dilution assay when used at a concentration of 0.1% w/v.⁴ Dietary administration of cholic acid (0.4% w/w) increases serum cholesterol levels, biliary phospholipid secretion, and fecal DCA levels in rats.⁵

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