ddGTP |
Katalog-Nr.GB20018
One-Click Copy Product Info
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ddGTP (2’, 3’-Dideoxyguanosine 5’-triphosphate) is a dideoxynucleotide used as a chain terminator in DNA sequencing.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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ddGTP (2’, 3’-Dideoxyguanosine 5’-triphosphate) is a dideoxynucleotide used as a chain terminator in DNA sequencing[1]. Unlike dGTP, ddGTP lacks hydroxyl groups at both the 2’ and 3’ positions of the ribose moiety. This structural feature prevents the formation of a 3’, 5’-phosphodiester bond after being incorporated by polymerase into a growing DNA strand, resulting in termination of DNA synthesis[2]. ddGTP is commonly employed in cycle sequencing, enzymatic mechanism studies, and the generation of RNA or DNA sequences that cannot be extended by polymerases or ligated by DNA ligases[3].
In vitro, ddGTP (＜1μM) strongly inhibits DNA polymerase α activity in the presence of Mn2+ by competing with the natural substrate dGTP for the enzyme’s binding site, exhibiting an inhibition constant (Ki) of 0.035μM[4]. ddGTP effectively inhibits the reverse transcriptase of human and simian immunodeficiency viruses (HIV and SIV), with Ki values of 0.009μM and 0.011μM, respectively[5]. Following incubation with TS30- and TS60-modified biosensors and Jurkat T cells for 3h in the presence of 0.2mM ddGTP, a subsequent 1h incubation in 10μM thiazole orange (TO) solution was performed. The mean fluorescence intensity (MFI) of the ddGTP-treated sensors (MFI=157) was significantly lower than that of the positive control group (MFI=261)[6]. Starch-TEPA/ddGTP complex (containing 1mg/mL starch-TEPA and 0.3mg/mL ddGTP) incubated with A549 cells at 37℃ for 4h exhibited high biological activity (99%), significantly surpassing the cell-killing effect of free ddGTP[7].
References:
[1] SANGER F, NICKLEN S, COULSON A R. DNA sequencing with chain-terminating inhibitors[J]. Proceedings of the National Academy of Sciences, 1977, 74(12): 5463-5467.
[2] ALPHEY L. Chain Termination (Sanger Dideoxy) Method[M]// DNA Sequencing. New York: Garland Science, 2023: 15-25.
[3] EREN K, TAKTAKOĞLU N, PIRIM I. DNA sequencing methods: from past to present[J]. The Eurasian Journal of Medicine, 2022, 54(Suppl 1): S47.
[4] ONO K, NAKANE H. Utilization of 2', 3'-dideoxyguanosine 5'-triptiosphate as an inhibitor and substrate for DNA polymerase α[J]. Biomedicine & Pharmacotherapy, 1990, 44(2): 115-121.
[5] WU J C, CHERNOW M, BOEHME R E, et al. Kinetics and inhibition of reverse transcriptase from human and simian immunodeficiency viruses[J]. Antimicrobial Agents and Chemotherapy, 1988, 32(12): 1887-1890.
[6] DÍAZ-CARTAGENA D C, HERNÁNDEZ-CANCEL G, BRACHO-RINCÓN D P, et al. Label-free telomerase activity detection via electrochemical impedance spectroscopy[J]. ACS Omega, 2019, 4(16): 16724-16732.
[7] KANBER E, YAMADA H, LORETZ B, et al. Design of polyamine-grafted starches for nucleotide analogue delivery: in vitro evaluation of the anticancer activity[J]. Bioconjugate Chemistry, 2016, 27(10): 2431-2440.
| Cas No. | SDF | ||
| Formula | C10H16N5O12P3 (free acid) | M.Wt | 491.1 (free acid) |
| Löslichkeit | Storage | Store at -20°C or below | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0362 mL | 10.1812 mL | 20.3625 mL |
| 5 mM | 407.2 μL | 2.0362 mL | 4.0725 mL |
| 10 mM | 203.6 μL | 1.0181 mL | 2.0362 mL |
Quality Control & SDS
- View current batch:
- Purity: >97.50% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)