Fluorescein-12-UTP, 10mM Sodium Solution |
Katalog-Nr.GB20111
One-Click Copy Product Info
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Fluorescein-12-UTP ist ein UTP-Analogon von Fluorescein, das UTP bei der RNA-Markierung in In-vitro-Transkriptionsreaktionen ersetzen kann.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 185843-23-6
Sample solution is provided at 25 µL, 10mM.
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Fluorescein-12-UTP, 10mM Sodium solution, a fluorescently labeled nucleotide analog that serves as a substrate for RNA polymerases such as SP6, T3, and T7, is widely used for RNA labeling and detection, with an Ex/Em of 496nm/520nm[1,2]. In in vitro transcription reactions, Fluorescein-12-UTP, 10 mM Sodium solution can replace uridine triphosphate (UTP) and be incorporated into RNA via an amide bond, thereby enabling fluorescent labeling of RNA. Fluorescein-12-UTP, 10mM Sodium solution is suitable for applications such as cellular fluorescence imaging, fluorescence in situ hybridization (FISH), bioimaging, and diagnostics[3,4,5].
References:
[1] CLARKE P A. Labeling and purification of RNA synthesized by in vitro transcription[M]//RNA-Protein Interaction Protocols. Totowa, NJ: Humana Press, 1999: 1-10.
[2] SEETHALA R. Homogeneous assays for high-throughput and ultrahigh-throughput screening[M]//Handbook of Drug Screening. New York: CRC Press, 2001: 90-149.
[3] HUGHES S C, DREAN B S L, LIVNE-BAR I, et al. Fluorescence in situ hybridization in whole-mount Drosophila embryos[J]. Biotechniques, 1996, 20(5): 748-750.
[4] YANG L, ELLINGTON A D. 2 Prospects for the De Novo Design of Nucleic Acid[J]. Fluorescence Sensors and Biosensors, 2005: 5.
[5] BELLINGHAM J, CHAURASIA S S, MELYAN Z, et al. Evolution of melanopsin photoreceptors: discovery and characterization of a new melanopsin in nonmammalian vertebrates[J]. PLoS Biology, 2006, 4(8): e254.
| Cas No. | 185843-23-6 | SDF | |
| Formula | C39H41N4O22P3 | M.Wt | 1010.68 |
| Löslichkeit | Storage | Store at -20°C,protect from light | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 989.4 μL | 4.9472 mL | 9.8943 mL |
| 5 mM | 197.9 μL | 989.4 μL | 1.9789 mL |
| 10 mM | 98.9 μL | 494.7 μL | 989.4 μL |
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solution
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)