Oleic acid-FITC (Synonyms: Oleic acid-FITC; FITC-OA; ) |
Katalog-Nr.GC26188
One-Click Copy Product Info
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Oleic acid-FITC is one of fatty acid lipid fluorescein conjugates that is highly hydrophobic..（Ex/Em: 494/515 nm）
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Oleic acid-FITC is one of SLT's fatty acid lipid fluorescein conjugates that is highly hydrophobic. Oleic acid is an un-saturated C18 fatty acid that can interact well with hydrophobic cell membrane or lysome membrane. Fluorescein modified oleic acid has strong green fluorescence with exitation/emission at 494 nm/515 nm. Oleic acid-FITC thus can be an excellent membrane imaging agent.
Appearance: Orange/yellow solid;
Ex/Em: 494/515 nm;
Solubility: Soluble in DMSO;
1.PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 C after preparation. Avoid repeated freeze-thaw cycles
Oleic acid-FITC stock solution
Prepare a 5 to 10 mM stock solution by dissolving an appropriate amount of Oleic acid-FITC in DMSO.
Note: For example, to prepare a 5 mM DMSO stock solution dissolve 1 mg of the Oleic acid-FITC in 235 μL of DMSO.
2.PREPARATION OF WORKING SOLUTION
Oleic acid-FITC working solution
Prepare a 5 to 10 μM Oleic acid-FITC working solution by diluting the stock solution in HHBS.
Note: Prepare a fresh working solution before use
3.SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be used as a guideline. lf needed, optimize the protocol to achieve the desired results.
1. Culture cells as desired.
2. Remove cell culture medium.
Note: Cell culture medium must be removed since it may interfere with the conjugate staining.
3. Add 100 μL of Oleic acid-FITC working solution per well.
4. Incubate the cells in the dark for 30 minutes at roomtemperature.
5. Remove the Oleic acid-FITC working solution and wash cells twice with HHBS.
6. Add HHBS buffer to the cells and observe the cells using a fluorescence microscope with a FITC filter set.
7. Optional: The cells can be fixed after staining.
| Cas No. | SDF | ||
| Überlieferungen | Oleic acid-FITC; FITC-OA; | ||
| Formula | C41H51N3O6S | M.Wt | 713.934 |
| Löslichkeit | Organic solvents/water | Storage | -20°C; protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.4007 mL | 7.0034 mL | 14.0069 mL |
| 5 mM | 280.1 μL | 1.4007 mL | 2.8014 mL |
| 10 mM | 140.1 μL | 700.3 μL | 1.4007 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A Solid or powder
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)