Nigerose |
Katalog-Nr.GF05558
One-Click Copy Product Info
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Nigerose is a disaccharide composed of two glucose units linked by an α-1,3 glycosidic bond.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 497-48-3
Sample solution is provided at 25 µL, 10mM.
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Nigerose is a disaccharide composed of two glucose units linked by an α-1,3 glycosidic bond. Nigerose possesses immunoenhancing activity, acts as a prebiotic to promote the growth of beneficial intestinal flora, and may exert antioxidant effects by activating the Nrf2 pathway. Nigerose can be used in research related to functional foods, prebiotic development, enzymology studies, nanodrug delivery, and immunomodulation[1-4].
In vitro, Caco-2/HepG2 co-cultured cells were treated with Nigerose (14mM) for 24 hours. Nigerose significantly increased resazurin conversion in Caco-2 cells. When co-exposed with a 0.5mM palmitic/oleic fatty acid mixture, Nigerose showed a small but non-significant effect on fat accumulation[5]. HL-60 cells were treated with Nigerose (300μM) for 1 or 24 hours. Nigerose increased cellular glutathione reductase activity, decreased lactate dehydrogenase activity and lipid oxidation, and alleviated protein carbonylation or oxidation[6].
In vivo, C57BL/6 mice were treated with Nigerose (2.57% diet or 0.18% drinking water) together with nigerosyl glucose and nigerosyl maltose for 14 days. Nigerose significantly enhanced L. plantarum L-137-induced IL-12 production and IL-2-stimulated IFN-γ production, and improved the resistance of mice to 5-fluorouracil-induced endogenous infection[7]. BDF1 mice were treated with Nigerose (0.08mg; intraperitoneal injection) together with nigerosyl glucose and nigerosyl maltose for 17 hours, or C57BL/6 mice were treated with Nigerose (0.20% drinking water orally) together with nigerosyl glucose and nigerosyl maltose for 7 days. Nigerose significantly inhibited EL-4 tumor growth in the liver[8].
References:
[1] Ofman TP, Küllmer F, van der Marel GA, et al. An Orthogonally Protected Cyclitol for the Construction of Nigerose- and Dextran-Mimetic Cyclophellitols. Org Lett. 2021 Dec 17;23(24):9516-9519.
[2] Franceus J, Dhaene S, Decadt H, et al. Rational design of an improved transglucosylase for production of the rare sugar nigerose. Chem Commun (Camb). 2019 Apr 11;55(31):4531-4533.
[3] McAlister D, Doyle RJ, Taylor KG. Inhibition by maltose, isomaltose, and nigerose of the synthesis of high-molecular-weight D-glucans by the D-glucosyltransferases of Streptococcus sobrinus. Carbohydr Res. 1989 Apr 1;187(1):131-8.
[4] Contour-Galcera MO, Guillot JM, Ortiz-Mellet C, et al. Synthesis of sulfur-linked analogues of nigerose, laminarabiose, laminaratriose, gentiobiose, gentiotriose, and laminaran trisaccharide Y. Carbohydr Res. 1996 Feb 7;281(1):99-118.
[5] van Laar A, Grootaert C, Van Nieuwerburgh F, et al. Metabolism and Health Effects of Rare Sugars in a CACO-2/HepG2 Coculture Model. Nutrients. 2022 Jan 30;14(3):611.
[6] Al-Otaibi NAS, Cassoli JS, Martins-de-Souza D, et al. Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives. Gigascience. 2019 Mar 1;8(3):giy155.
[7] Murosaki S, Muroyama K, Yamamoto Y, et al. Immunopotentiating activity of nigerooligosaccharides for the T helper 1-like immune response in mice. Biosci Biotechnol Biochem. 1999 Feb;63(2):373-8.
[8] Murosak S, Muroyama K, Yamamoto Y, et al. Nigerooligosaccharides augments natural killer activity of hepatic mononuclear cells in mice. Int Immunopharmacol. 2002 Jan;2(1):151-9.
| Cell experiment [1]: | |
Cell lines | Caco-2 (human intestinal colorectal adenocarcinoma cells) and HepG2 (hepatoma G2 liver cells) coculture model |
Preparation Method | Caco-2 cells were seeded in 24-well transwell inserts and differentiated for at least 3 weeks. HepG2 cells were seeded at a density of 5×10⁴ cells per well in collagen-coated 24-well plates. Cells were cocultured for 24h followed by a 24h exposure. Caco-2 and HepG2 cells were cultured in DMEM supplemented with 10% heat-inactivated FBS, 1% non-essential amino acids and 1% penicillin/streptomycin at 37°C and 10% CO₂. |
Reaction Conditions | 14mM; 24h |
Applications | Nigerose significantly increased resazurin conversion in Caco-2 cells. In the coculture model with dietary fatty acids, there was a non-significant trend towards higher median fat accumulation with Nigerose compared to control. |
| Animal experiment [2]: | |
Animal models | BDF1 and C57BL/6 mice |
Preparation Method | BDF1 mice were intraperitoneally injected with Nigerose 17 hours prior to intravenous inoculation with 1×10⁶ EL-4 tumor cells. C57BL/6 mice were given Nigerose solution as drinking fluid for 7 days before tumor inoculation. |
Dosage form | 0.08mg; i.p.; single injection; 0.20% in drinking water; oral; 7 days |
Applications | Nigerose significantly inhibited EL-4 tumor growth in the liver and improved the survival curve of tumor-bearing mice. |
References: | |
| Cas No. | 497-48-3 | SDF | |
| Formula | C12H22O11 | M.Wt | 342.3 |
| Löslichkeit | Storage | Store at -20°C, stored under nitrogen | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9214 mL | 14.6071 mL | 29.2141 mL |
| 5 mM | 584.3 μL | 2.9214 mL | 5.8428 mL |
| 10 mM | 292.1 μL | 1.4607 mL | 2.9214 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >97.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)