GRGDSP |
Katalog-Nr.GC33323
One-Click Copy Product Info
|
GRGDSP, ein synthetisches lineares RGD-Peptid, ist ein Integrin-Inhibitor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 91037-75-1
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
GRGDSP, a synthetic linear RGD peptide, is an integrin inhibitor [1]. GRGDSP can induce cells to become round and activate matrix metalloproteinase-2 (MMP-2), thereby increasing the levels of membrane-bound precursors and active forms of MMP-1 and MMP-2 [2]. GRGDSP has been used to modify the surface of cardiovascular implants such as vascular grafts to promote endothelialization [3].
In vitro, GRGDSP treatment (5mM) for 48h significantly inhibited the viability of rabbit chondrocytes and synovial cells, accompanied by an increase in Caspase-3 activity[4]. 10nM of GRGDSP treatment for 24 hours significantly promoted the migration of human foreskin fibroblasts (HFF)[5].
In vivo, GRGDSP treatment via administration (1mg/kg/day) at the injured site for 14 days significantly delayed the remyelination in Dhhcre/+;Ninj2fl/fl mice and reduced motor coordination and locomotion in mice [6]. A single arterial infusion of 0.25mg of GRGDSP combined with transarterial chemoembolization (TACE) inhibited the invasive growth and metastasis of tumor cells in rats with hepatic carcinoma within 12 days[7].
References:
[1] Qian J, Oppermann E, Tran A, et al. Transarterial administration of integrin inhibitor loaded nanoparticles combined with transarterial chemoembolization for treating hepatocellular carcinoma in a rat model[J]. World Journal of Gastroenterology, 2016, 22(21): 5042.
[2] Teti A, Farina A R, Villanova I, et al. Activation of MMP‐2 by human GCT23 giant cell tumour cells induced by osteopontin, bone sialoprotein and GRGDSP peptides is RGD and cell shape change dependent[J]. International journal of cancer, 1998, 77(1): 82-93.
[3] Patel S, Tsang J, Harbers G M, et al. Regulation of endothelial cell function by GRGDSP peptide grafted on interpenetrating polymers[J]. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 2007, 83(2): 423-433.
[4] Matsuki K, Sasho T, Nakagawa K, et al. RGD peptide-induced cell death of chondrocytes and synovial cells[J]. Journal of Orthopaedic Science, 2008, 13(6): 524-532.
[5] Knapp D M, Helou E F, Tranquillo R T. A fibrin or collagen gel assay for tissue cell chemotaxis: assessment of fibroblast chemotaxis to GRGDSP[J]. Experimental cell research, 1999, 247(2): 543-553.
[6] Sun Y, Chen X, Yue C, et al. Ninj2 regulates Schwann cells development by interfering laminin-integrin signaling[J]. Theranostics, 2022, 12(17): 7307.
[7] Qian J, Yin J, Liang H, et al. Experimental study on transarterial administration of GRGDSP combined with transarterial chemoembolization in rats with hepatic carcinoma[J]. Cardiovascular and interventional radiology, 2008, 31(2): 377-382.
| Cell experiment [1]: | |
Cell lines | Rabbit synovial cells |
Preparation Method | Rabbit synovial cells were cultivated in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100units/ml penicillin, and 100mg/ml streptomycin at 37°C in an incubator with 5% CO2. Cells were seeded in a 12-well plate at a density of 2×105 cells/well for 24h. Cells were treated with 5mM GRGDSP and serum-free medium. After 48h of treatment, the cell viability was determined. |
Reaction Conditions | 5mM; 48h |
Applications | GRGDSP treatment significantly inhibited cell viability of rabbit synovial cells. |
| Animal experiment [2]: | |
Animal models | Male Wistar rats |
Preparation Method | Male Wistar rats (180-220g) were maintained in a controlled environment at 22±3°C and 60% relative humidity under a 12h light/dark cycle, and were given free access to standard rodent nutrition and water. Walker-256 tumor tissue was recovered from a propagation animal 10 days after subcutaneous implantation and cut into small cubes approximately 2mm3 in volume. The rats were anesthetized, the abdominal wall was opened, and a small subcapsular incision was made on the left lateral lobe of the liver. The tumor fragment was gently placed into the pocket and covered with a small piece of a cotton swab on the liver surface. The abdominal wall was subsequently closed. Using a binocular operative microscope, a PE-10 polyethylene microcatheter (inner diameter 0.28mm, outer diameter 0.61mm) was retrogradely inserted via the gastroduodenal artery into the hepatic artery. The therapeutic agents were mixed and then injected into the hepatic artery within 20min. Each group received treatment according to the following protocols: Group A (GRGDSP + TACE; n = 9): 0.05ml mitomycin (1mg/ml) + 0.1ml GRGDSP (2.5mg/ml) + 0.05ml lipiodol + ligation of the proper hepatic artery; Group B (TACE alone; n = 8): 0.05ml mitomycin (1 mg/ml) + 0.05ml lipiodol + ligation of the proper hepatic artery; Group C (normal saline; n = 9): 0.2ml 0.9% NaCl. Twelve days after interventional therapy, MRI examination was again performed, and the post-treatment volume of the hepatic tumor (V2) was measured. |
Dosage form | 2.5mg/ml (0.1ml) for once; hepatic artery administration |
Applications | GRGDSP treatment significantly inhibited combined with TACE inhibited the hepatic carcinoma growth in rats. |
References: | |
| Cas No. | 91037-75-1 | SDF | |
| Canonical SMILES | Gly-Arg-Gly-Asp-Ser-Pro | ||
| Formula | C22H37N9O10 | M.Wt | 587.58 |
| Löslichkeit | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.7019 mL | 8.5095 mL | 17.019 mL |
| 5 mM | 340.4 μL | 1.7019 mL | 3.4038 mL |
| 10 mM | 170.2 μL | 850.9 μL | 1.7019 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 40 reference(s) in Google Scholar.)