ICG-carboxylic acid |
Katalog-Nr.GC63438
One-Click Copy Product Info
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ICG-CarbonsÄure ist eine im nahen Infrarot (NIR) fluoreszierende Sonde.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 181934-09-8
Sample solution is provided at 25 µL, 10mM.
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ICG-carboxylic acid is a near-infrared (NIR) fluorescent probe based on indocyanine green (ICG), characterized by good water solubility, low toxicity, and stability[1]. As a carboxylated form of ICG, ICG-carboxylic acid has an absorption/emission wavelength of approximately 785/800nm[2]. The carboxylic acid group is activated by the EDC/NHS reaction, making ICG-carboxylic acid readily conjugatable with other biomolecules, such as proteins, peptides, and nucleic acids[3]. ICG-carboxylic acid is commonly used in biomedical research fields, including tissue imaging, photothermal therapy, tumor detection, and drug delivery systems[4-7].
References:
[1] Taylor, J. S., Zeki, J., Ikegaki, N., Chen, L. L., & Chiu, B. (2018). Combined application of Indocyanine green (ICG) and laser lead to targeted tumor cell destruction. Journal of pediatric surgery, 53(12), 2475–2479.
[2] Mačianskienė, R., Almanaitytė, M., Treinys, R., Navalinskas, A., Benetis, R., & Jurevičius, J. (2017). Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart. Scientific reports, 7(1), 7983.
[3] Wang, P., Wang, X., Luo, Q., Li, Y., Lin, X., Fan, L., Zhang, Y., Liu, J., & Liu, X. (2019). Fabrication of Red Blood Cell-Based Multimodal Theranostic Probes for Second Near-Infrared Window Fluorescence Imaging-Guided Tumor Surgery and Photodynamic Therapy. Theranostics, 9(2), 369–380.
[4] Kaplan-Marans, E., Fulla, J., Tomer, N., Bilal, K., & Palese, M. (2019). Indocyanine Green (ICG) in Urologic Surgery. Urology, 132, 10–17.
[5] Chen, Z., Zhao, P., Luo, Z., Zheng, M., Tian, H., Gong, P., Gao, G., Pan, H., Liu, L., Ma, A., Cui, H., Ma, Y., & Cai, L. (2016). Cancer Cell Membrane-Biomimetic Nanoparticles for Homologous-Targeting Dual-Modal Imaging and Photothermal Therapy. ACS nano, 10(11), 10049–10057.
[6] Shao, C., Li, Z., Zhang, C., Zhang, W., He, R., Xu, J., & Cai, Y. (2022). Optical diagnostic imaging and therapy for thyroid cancer. Materials today. Bio, 17, 100441.
[7] Lu, K., Pan, X., Zheng, J., Cheng, D., Zheng, L., & Zhang, X. (2024). Theranostic nanoparticles ZIF-8@ICG for pH/NIR-responsive drug-release and NIR-guided chemo-phototherapy against non-small-cell lung cancer. Journal of materials science. Materials in medicine, 35(1), 32.
Protocol for ICG-carboxylic acid in Cardiac Voltage-sensitive Imaging[1]
1. Animal preparation
Experiments were performed on Langendorff-perfused hearts from New Zealand White rabbits of both sexes weighing 3.8 ± 0.1kg (n = 9).
2. Cardiac perfusion
The heart was quickly cannulated through the aorta and attached to a Langendorff perfusion system with a constant pressure of ~80mmHg and perfused with oxygenated (100% O2) Tyrode’s solution (in mM: 135 NaCl, 5.4 KCl, 1.8 CaCl2, 0.9 MgCl2, 0.33 NaH2PO4, 10 glucose, and 10 HEPES; pH 7.4) at 37 ± 0.5°C.
The coronary flow rate was 39.1 ± 0.6mL/min. After allowing approximately 20–30 minutes for the preparation to equilibrate, the perfusion was switched to recirculation mode.
3. Heart staining
The heart was stained by adding increasing concentrations (from 0.03µM to 10µM) of the ICG-carboxylic acid dye to the perfusate.
4. Fluorescence excitation
Collimated LEDs were used for excitation (617nm, 660nm, 735nm, and 780nm; filtered at the corresponding wavelengths with band-pass filters).
5. Fluorescence detection
Emission was measured using band-pass and long-pass filters for various wavelengths (from 700nm to 905nm) with a cooled (−100°C) fast 14-bit EMCCD camera equipped with a 50-mm focal length objective and with a spectrometer (from 350nm to 1020nm).
6. Data Collection
The anterior surface of the LV was imaged, and the field of view was 20 × 20mm. Optical movies were acquired at a sampling rate of 500Hz with a resolution of 128 × 128 pixels using imaging software.
7. Data Analysis
The ICG-carboxylic acid fluorescence data obtained were used for further analysis.
This protocol only provides a guideline, and should be modified according to your specific needs.
[1] Ma?ianskien?, R., Almanaityt?, M., Treinys, R., Navalinskas, A., Benetis, R., & Jurevi?ius, J. (2017). Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart. Scientific reports, 7(1), 7983.
| Cas No. | 181934-09-8 | SDF | |
| Formula | C45H50N2O5S | M.Wt | 730.95 |
| Löslichkeit | DMSO : 50 mg/mL (68.40 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3681 mL | 6.8404 mL | 13.6808 mL |
| 5 mM | 273.6 μL | 1.3681 mL | 2.7362 mL |
| 10 mM | 136.8 μL | 684 μL | 1.3681 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 37 reference(s) in Google Scholar.)