Janelia Fluor 549, SE (Synonyms: JF549, SE; JF549, NHS) |
| Katalog-Nr.GC50359 |
Janelia Fluor 549, SE (JF549, SE) ist ein Fluoreszenzfarbstoff mit einem Absorptionsmaximum (λab (max)) von 549 nm und einem Emissionsmaximum (λem (max)) von 571 nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1811539-32-8
Sample solution is provided at 25 µL, 10mM.
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Yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169. We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID:25599551 |Deng et al (2015) CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc.Natl.Acad.Sci.USA. 112 11870 PMID:26324940 |Knight et al (2015) Dynamics of CRISPR-Cas9 genome interrogation in living cells. Science 350 823 PMID:26564855 |Legant et al (2016) High-density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID:26950745
Average Rating: 5 (Based on Reviews and 23 reference(s) in Google Scholar.)
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