Penicillin G Sodium |
Katalog-Nr.GC17181
One-Click Copy Product Info
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Penicillin G Natrium ist ein typisches β-Lactam-Antibiotikum.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 69-57-8
Sample solution is provided at 25 µL, 10mM.
Penicillin G Sodium is an antibiotic containing a β-lactam ring against sensitive organisms [1]. Penicillin G Sodium binds to penicillin-binding proteins (PBPs) of bacteria which are involved in cell wall synthesis and thus inhibit bacterial cell wall synthesis [2]. Penicillin G Sodium has been widely used to kill Staphylococcus aureus, and to develop a combined therapy with lactoferrin to induce the lysis of various bacteria and changes in the cell wall[3].
In vitro, Penicillin G Sodium treatment (150U/ml) for 48 hours led to the formation of abnormal Chlamydia trachomatis (CT) inclusions within HeLa cells infected with CT and without causing cytotoxicity, and altered the mRNA expression profile of CT[4].
In vivo, Penicillin G Sodium treatment (160mg/kg; s.c.) combined with gentamicin (10mg/kg; s.c.) twice daily for 5 days can significantly increase the survival rate of mice with penicillin-resistant pneumococcal pneumonia [5].
References:
[1] Chu L, Zhuang S, Wang J. Degradation kinetics and mechanism of penicillin G in aqueous matrices by ionizing radiation[J]. Radiation Physics and Chemistry, 2018, 145: 34-38.
[2] Li D, Yang M, Hu J, et al. Determination of penicillin G and its degradation products in a penicillin production wastewater treatment plant and the receiving river[J]. Water Research, 2008, 42(1-2): 307-317.
[3] Diarra M S, Lacasse P, Deschênes E, et al. Ultrastructural and cytochemical study of cell wall modification by lactoferrin, lactoferricin and penicillin G against Staphylococcus aureus[J]. Journal of electron microscopy, 2003, 52(2): 207-215.
[4] Wang M, Qiu X, Xu Y, et al. Impacts of penicillin G on Chlamydia trachomatis serovar D and host HeLa cells in genital infections[J]. Archives of Microbiology, 2025, 207(7): 164.
[5] Tateda K, Matsumoto T, Miyazaki S, et al. Efficacy of β-lactam antibiotics combined with gentamicin against penicillin-resistant pneumococcal pneumonia in CBA/J mice[J]. Journal of Antimicrobial Chemotherapy, 1999, 43(3): 367-371.
| Cell experiment [1]: | |
Cell lines | HeLa cells |
Preparation Method | HeLa cell suspensions (5×104 cells/mL) were seeded into 6-well plates (2ml per well). Upon reaching a confluence of 80%, the culture medium was removed. Then the cells were treated with 30µg/ml of diethylaminoethyl dextran (DEAE-D) for 30min. Afterwards, the cells were cultured with serum-free Dulbecco’s modified Eagle’s medium (DMEM). The D/UW-3/CX strain of CT, containing an inclusion forming unit (IFU) concentration of 4.442×106 IFU/ml, was then inoculated onto the cells, followed by incubating at 37°C with 5% CO2 for 1h. The cells were then centrifuged at 500×g for 1h at 32°C, and the supernatant was removed. Fresh DMEM containing Penicillin G Sodium at a final concentration of 150U/ml was added to the wells and cultured for 48h. Cells with aberrant inclusions were selected using a microscope for further analysis. Cells were collected in 1.5ml tubes and centrifuged at 250×g for 10min at 32°C, fixed with glutaraldehyde, and subsequently examined using transmission electron microscopy (TEM). |
Reaction Conditions | 150U/ml; 48h |
Applications | Penicillin G Sodium treatment significantly induced formation of abnormal CT inclusions within HeLa cells. |
References: | |
| Cas No. | 69-57-8 | SDF | |
| Chemical Name | sodium;(2S,5R,6R)-3,3-dimethyl-7-oxo-6-[(2-phenylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate | ||
| Canonical SMILES | CC1(C(N2C(S1)C(C2=O)NC(=O)CC3=CC=CC=C3)C(=O)[O-])C.[Na+] | ||
| Formula | C16H18N2O4S.Na | M.Wt | 356.37 |
| Löslichkeit | ≥ 13.7mg/mL in DMSO | Storage | Store at -20°C,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8061 mL | 14.0304 mL | 28.0607 mL |
| 5 mM | 561.2 μL | 2.8061 mL | 5.6121 mL |
| 10 mM | 280.6 μL | 1.403 mL | 2.8061 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 1 reference(s) in Google Scholar.)