Tris-NTA |
Katalog-Nr.GC64727
One-Click Copy Product Info
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Tris-NTA ist ein His-markierter Proteinligand, der verwendet werden kann, um His-markierte Proteine zu binden.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 862778-60-7
Sample solution is provided at 25 µL, 10mM.
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Tris-NTA is a His-tagged protein ligand that can be used to bind His-tagged proteins[1]. Tris-NTA is a high-affinity metal chelator composed of three nitrotriacetic acid (NTA) groups connected by a tris(hydroxymethyl)aminomethane (Tris) backbone. Tris-NTA can specifically bind to hexahistidine-tagged (His-tag) proteins and stably chelate divalent metal ions (such as Ni2+, Co2+)[2, 3]. Tris-NTA has higher binding stability than single NTA ligands[4]. Tris-NTA can be chemically modified in a variety of ways (such as coupling fluorescent groups, biotin or nanocarriers), making it suitable for applications in targeted drug delivery and molecular diagnostics[5, 6].
References:
[1] Zhu L, Chang Y, Li Y, et al. Biosensors based on the binding events of nitrilotriacetic acid–metal complexes[J]. Biosensors, 2023, 13(5): 507.
[2] Auer S, Azizi L, Faschinger F, et al. Stable immobilisation of His-tagged proteins on BLI biosensor surface using cobalt[J]. Sensors and Actuators B: Chemical, 2017, 243: 104-113.
[3] Brellier M, Barlaam B, Mioskowski C, et al. Insight into the Complexation Mode of Bis (nitrilotriacetic acid)(NTA) Ligands with Ni2+ Involved in the Labeling of Histidine‐Tagged Proteins[J]. Chemistry–A European Journal, 2009, 15(46): 12689-12701.
[4] Lata S, Reichel A, Brock R, et al. High-affinity adaptors for switchable recognition of histidine-tagged proteins[J]. Journal of the American Chemical Society, 2005, 127(29): 10205-10215.
[5] Bartoschik T, Galinec S, Kleusch C, et al. Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis[J]. Scientific reports, 2018, 8(1): 4977.
[6] You C, Piehler J. Multivalent chelators for spatially and temporally controlled protein functionalization[J]. Analytical and bioanalytical chemistry, 2014, 406: 3345-3357.
| Cas No. | 862778-60-7 | SDF | |
| Formula | C43H68N8O22 | M.Wt | 1049.04 |
| Löslichkeit | H2O : ≥ 100 mg/mL (95.33 mM); DMSO : 50 mg/mL (47.66 mM; Need ultrasonic) | Storage | Store at 2-8°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 953.3 μL | 4.7663 mL | 9.5325 mL |
| 5 mM | 190.7 μL | 953.3 μL | 1.9065 mL |
| 10 mM | 95.3 μL | 476.6 μL | 953.3 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)