α-MSH (Synonyms: α-Melanocyte-Stimulating Hormone, a-MSH) |
Catalog No.GC14374
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Alpha-melanocyte-stimulating hormone (α-MSH) est un neuropeptide aux propriétés immunomodulatrices, produit par le traitement poste-traductionnel de la proproiomélanocortine (POMC).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 581-05-5
Sample solution is provided at 25 µL, 10mM.
Alpha-melanocyte-stimulating hormone (α-MSH) est un neuropeptide aux propriétés immunomodulatrices, produit par le traitement poste-traductionnel de la proproiomélanocortine (POMC). α-MSH se lie au récepteur MC1R sur les mélanocytes pour stimuler la sécrétion de mélanine, favoriser la formation de dentrites mélanocytaires et protéger les mélanocytes. α-MSH exerce un effet anti-inflammatoire puissant sur les cellules immunitaires. De plus, il a été démontré que α-MSH inhibe la production d'IFN-γ dans les lymphocytes T stimulés par un antigène [1-3].α-MSH-(1 µM; 72h) stimule la production de mélanine dans les cellules de mélanome de B16[4].α-MSH(0-10 ng/mL; 5-10min)renforce les réponses protectrices sous conditions de stress telles que les UV en augmentant la production de mélanine et en réduisant l'apoptose médiée par les mitochondries [5]. α-MSH(100 nM; 48 h) a augmenté l'activité de la tyrosinase et la teneur en mélanine respectivement de 56.5% et 61.7%, et a modifié le niveau des métabolites dans les cellules de mélanome [6].
α-MSH (1mg / kg; i.v;12 h) peut augmenter la charge bactérienne chez les souris soumises à une occlusion de l'artère cérébrale moyenne (MCAO)[7]. α-MSH(500 nM ; i.p;0-6 h) favorise l'oxydation des acides gras dans le tissu adipeux (WAT) et le tissu adipeux brun (BAT)[8].
References:
[1]. Ichiyama T, Sato S, et,al. The neuroimmunomodulatory peptide alpha-MSH. Ann N Y Acad Sci. 2000;917:221-6. doi: 10.1111/j.1749-6632.2000.tb05386.x. PMID: 11268347.
[2]. Li C, Wu M, et,al. α- MSH Plays Anti-inflammatory and Anti-fungal Role in Aspergillus Fumigatus Keratitis. Curr Eye Res. 2022 Mar;47(3):343-351. doi: 10.1080/02713683.2021.2006235. Epub 2021 Dec 12. PMID: 34766863.
[3]. Schulte-HerbrÜggen O, Quarcoo D, et,al. Alpha-MSH promotes spontaneous post-ischemic pneumonia in mice via melanocortin-receptor-1. Exp Neurol. 2008 Apr;210(2):731-9. doi: 10.1016/j.expneurol.2008.01.006. Epub 2008 Jan 19. PMID: 18304533.
[4]. Song HS, Sim SS. Acteoside inhibits alpha-MSH-induced melanin production in B16 melanoma cells by inactivation of adenyl cyclase. J Pharm Pharmacol. 2009 Oct;61(10):1347-51. doi: 10.1211/jpp/61.10.0011. PMID: 19814867.
[5]: Choudhary R, Sharma A, et,al. Role of alpha-melanocyte stimulating hormone (α-MSH) in modulating the molecular mechanism adopted by melanocytes of Bos indicus under UVR stress. Mol Cell Biochem. 2020 Feb;465(1-2):141-153. doi: 10.1007/s11010-019-03674-w. Epub 2019 Dec 10. PMID: 31823188.
[6]. Seo SH, Jo JK, et,al. Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells. Molecules. 2020 Jul 26;25(15):3384. doi: 10.3390/molecules25153384. PMID: 32722640; PMCID: PMC7436294.
[7]. Schulte-HerbrÜggen O, Quarcoo D, et,al. Alpha-MSH promotes spontaneous post-ischemic pneumonia in mice via melanocortin-receptor-1. Exp Neurol. 2008 Apr;210(2):731-9. doi: 10.1016/j.expneurol.2008.01.006. Epub 2008 Jan 19. PMID: 18304533.
[8]. Gan L, Liu Z, et,al. α-MSH and Foxc2 promote fatty acid oxidation through C/EBPβ negative transcription in mice adipose tissue. Sci Rep. 2016 Nov 7;6:36661. doi: 10.1038/srep36661. PMID: 27819350; PMCID: PMC5098202.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Les cellules de mélanome B16 |
Méthode de préparation | Les cellules traitées avec α-MSH dans des boîtes de culture (60 mm) ont été incubées pendant 72 h à 37℃ dans une chambre à CO2. |
Conditions de réaction | 1 µM; 72h |
Domaines d'application | α-MSH- stimule la production de mélanine dans les cellules de mélanome B16. |
| Expériences animales [2]: | |
Modèles animaux | Souris mâles C57BL/6J âgées de trois semaines |
Méthode de préparation | Les souris ont reçu par injection intrapéritonéale soit du PBS soit de α-MSH (500 nM), une fois par jour pendant deux jours avant le cycle obscur. Le tissu adipeux inguinal (iWAT) a été prélevé 0.5 h, 1 h, 2 h, 4 h et 6 h après la dernière injection dans le groupe traité. |
Forme de dosage | 500 nM ; i.p;0-6 h |
Domaines d'application | α-MSH favorise l'oxydation des acides gras dans le tissu adipeux blanc (WAT) et le tissu adipeux brun (BAT). |
| References: [1]. Song HS, Sim SS. Acteoside inhibits alpha-MSH-induced melanin production in B16 melanoma cells by inactivation of adenyl cyclase. J Pharm Pharmacol. 2009 Oct;61(10):1347-51. doi: 10.1211/jpp/61.10.0011. PMID: 19814867. | |
| Cas No. | 581-05-5 | SDF | |
| Synonymes | α-Melanocyte-Stimulating Hormone, a-MSH | ||
| Chemical Name | (4S,5Z,7S,8Z,10S,11Z,13S,14Z,16S,17Z,20Z,22S)-7-((1H-imidazol-5-yl)methyl)-16-((1H-indol-3-yl)methyl)-26-amino-10-benzyl-13-(3-guanidinopropyl)-5,8,11,14,17,20-hexahydroxy-22-((S)-2-((Z)-hydroxy(((S)-1-hydroxy-1-imino-3-methylbutan-2-yl)imino)methyl)pyrro | ||
| Canonical SMILES | CC([C@@](/N=C(O)/[C@]1([H])CCCN1C([C@](/N=C(O)/C/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/C)([H])CO)([H])CC2=CC=C(O)C=C2)([H])CO)([H])CCSC)([H])CCC(O)=O)([H])CC3=CN=CN3)([H | ||
| Formula | C77H109N21O19S | M.Wt | 1664.9 |
| Solubility | 25mg/ml in Water | Storage | Desiccate at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 600.6 μL | 3.0032 mL | 6.0064 mL |
| 5 mM | 120.1 μL | 600.6 μL | 1.2013 mL |
| 10 mM | 60.1 μL | 300.3 μL | 600.6 μL |
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Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)