α-MSH (Synonyms: α-Melanocyte-Stimulating Hormone, a-MSH) |
カタログ番号GC14374
One-Click Copy Product Info
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α-メラノサイト刺激ホルモン（Alpha-melanocyte-stimulating hormone）(α-MSH)はプロオピオメラノコルチン(POMC)の翻訳後修飾により産生される免疫調節特性を持つ神経ペプチドである
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 581-05-5
Sample solution is provided at 25 µL, 10mM.
α-メラノサイト刺激ホルモン（Alpha-melanocyte-stimulating hormone）(α-MSH)はプロオピオメラノコルチン(POMC)の翻訳後修飾により産生される免疫調節特性を持つ神経ペプチドである。α-MSHはメラノサイト上のMC1R受容体に結合し、メラニン分泌を刺激し、メラノサイト樹状突起の形成を促進し、メラノサイトを保護する。α-MSHは免疫細胞に対し効果的な抗炎症作用をを示している。さらに、α-MSHは抗原刺激T細胞におけるIFN-γ産生を阻害することが示されている。B16メラノーマ細胞においてメラニンの産生はα-MSH(1 µM; 72h)により刺激される。α-MSH(0-10 ng/mL; 5-10min)はメラニン産生を増加させ、ミトコンドリアを介したアポトーシスを減少させることによってUVRのようなストレス条件下での保護応答の役割を強化する。α-MSH(100 nM; 48 h)はチロシナーゼ活性を56.5%、メラニン含量を61.7%増加させた。
α-MSH (1mg / kg; i.v;12 h)は中大脳動脈閉塞（MCAO）マウスにおいて細菌負荷を増加させることができる。α-MSH(500 nM ; i.p;0-6 h)はWATおよびBATにおける脂肪酸酸化を促進させる。
References:
[1]. Ichiyama T, Sato S, et,al. The neuroimmunomodulatory peptide alpha-MSH. Ann N Y Acad Sci. 2000;917:221-6. doi: 10.1111/j.1749-6632.2000.tb05386.x. PMID: 11268347.
[2]. Li C, Wu M, et,al. α- MSH Plays Anti-inflammatory and Anti-fungal Role in Aspergillus Fumigatus Keratitis. Curr Eye Res. 2022 Mar;47(3):343-351. doi: 10.1080/02713683.2021.2006235. Epub 2021 Dec 12. PMID: 34766863.
[3]. Schulte-HerbrÜggen O, Quarcoo D, et,al. Alpha-MSH promotes spontaneous post-ischemic pneumonia in mice via melanocortin-receptor-1. Exp Neurol. 2008 Apr;210(2):731-9. doi: 10.1016/j.expneurol.2008.01.006. Epub 2008 Jan 19. PMID: 18304533.
[4]. Song HS, Sim SS. Acteoside inhibits alpha-MSH-induced melanin production in B16 melanoma cells by inactivation of adenyl cyclase. J Pharm Pharmacol. 2009 Oct;61(10):1347-51. doi: 10.1211/jpp/61.10.0011. PMID: 19814867.
[5]: Choudhary R, Sharma A, et,al. Role of alpha-melanocyte stimulating hormone (α-MSH) in modulating the molecular mechanism adopted by melanocytes of Bos indicus under UVR stress. Mol Cell Biochem. 2020 Feb;465(1-2):141-153. doi: 10.1007/s11010-019-03674-w. Epub 2019 Dec 10. PMID: 31823188.
[6]. Seo SH, Jo JK, et,al. Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells. Molecules. 2020 Jul 26;25(15):3384. doi: 10.3390/molecules25153384. PMID: 32722640; PMCID: PMC7436294.
[7]. Schulte-HerbrÜggen O, Quarcoo D, et,al. Alpha-MSH promotes spontaneous post-ischemic pneumonia in mice via melanocortin-receptor-1. Exp Neurol. 2008 Apr;210(2):731-9. doi: 10.1016/j.expneurol.2008.01.006. Epub 2008 Jan 19. PMID: 18304533.
[8]. Gan L, Liu Z, et,al. α-MSH and Foxc2 promote fatty acid oxidation through C/EBPβ negative transcription in mice adipose tissue. Sci Rep. 2016 Nov 7;6:36661. doi: 10.1038/srep36661. PMID: 27819350; PMCID: PMC5098202.
| 細胞実験[1]: | |
細胞株 | B16 メラノーマ細胞 |
準備方法 | 培養ディッシュ(60 mm) にα-MSHで処理した細胞をCO2チャンバーで37℃で72時間培養した |
反応条件 | 1 µM; 72h |
アプリケーション | B16メラノーマ細胞においてメラニンの産生はα-MSH(1 µM; 72h)により刺激される |
| 動物実験 [2]: | |
動物モデル | 3週齢C57BL/6Jオスマウス |
準備方法 | マウスにPBSまたはα-MSH (500 nM)をdark cycle前に1日1回で2日間腹腔内注射した。鼠径部白色脂肪組織（IWAT）は処置群最後の注射後の0.5時間、1時間、2時間、4時間、6時間に摘出した。 |
投与形態 | 500 nM ; i.p;0-6 h |
アプリケーション | α-MSH(500 nM ; i.p;0-6 h)はWATおよびBATにおける脂肪酸酸化を促進させる |
| References: [1]. Song HS, Sim SS. Acteoside inhibits alpha-MSH-induced melanin production in B16 melanoma cells by inactivation of adenyl cyclase. J Pharm Pharmacol. 2009 Oct;61(10):1347-51. doi: 10.1211/jpp/61.10.0011. PMID: 19814867. | |
| Cas No. | 581-05-5 | SDF | |
| 同義語 | α-Melanocyte-Stimulating Hormone, a-MSH | ||
| Chemical Name | (4S,5Z,7S,8Z,10S,11Z,13S,14Z,16S,17Z,20Z,22S)-7-((1H-imidazol-5-yl)methyl)-16-((1H-indol-3-yl)methyl)-26-amino-10-benzyl-13-(3-guanidinopropyl)-5,8,11,14,17,20-hexahydroxy-22-((S)-2-((Z)-hydroxy(((S)-1-hydroxy-1-imino-3-methylbutan-2-yl)imino)methyl)pyrro | ||
| Canonical SMILES | CC([C@@](/N=C(O)/[C@]1([H])CCCN1C([C@](/N=C(O)/C/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/C)([H])CO)([H])CC2=CC=C(O)C=C2)([H])CO)([H])CCSC)([H])CCC(O)=O)([H])CC3=CN=CN3)([H | ||
| Formula | C77H109N21O19S | M.Wt | 1664.9 |
| 溶解度 | 25mg/ml in Water | Storage | Desiccate at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 600.6 μL | 3.0032 mL | 6.0064 mL |
| 5 mM | 120.1 μL | 600.6 μL | 1.2013 mL |
| 10 mM | 60.1 μL | 300.3 μL | 600.6 μL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
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Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)