α-Factor Mating Pheromone, yeast (Mating Factor α) |
カタログ番号GC30587
One-Click Copy Product Info
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α-Factor Mating Pheromone, yeast (Mating Factor α)は、Saccharomyces cerevisiaeのMATα型細胞によって連続的に合成および分泌される13アミノ酸ペプチドホルモンであり、MATA型細胞に特異的に作用することができる。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 59401-28-4
Sample solution is provided at 25 µL, 10mM.
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α-Factor Mating Pheromone, yeast (Mating Factor α)は、Saccharomyces cerevisiaeのMATα型細胞によって連続的に合成および分泌される13アミノ酸ペプチドホルモンであり、MATA型細胞に特異的に作用することができる[1]。α-Factor Mating Pheromone, yeast (Mating Factor α)はMATA細胞の表面上のGたんぱく質の共役受容体Ste2ｐ結合し、細胞周期停止、形態学的な変化および交配関連の遺伝子の発現を引き起こす。この因子は結合（KD =0.3μm）のために放射性の標識³⁵S-α-ファクターと競合できる。α-Factor Mating Pheromone, yeast (Mating Factor α)の熱感受性Ste2変異体への結合は熱的な不安定であり、ほかの変異体（Ste4，Ste5，Ste7，Ste11和Ste12）との結合はそうではない[2]。α-Factor Mating Pheromone, yeast (Mating Factor α)は酵母を誘発して、交配状態に入るためによく使用され、外来のたんぱく質の分泌と発現に広く使用している[3]。
References:
[1]. Naider F, Becker JM. The α-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors. Peptides. 2004 Sep 1; 25(9): 1441-1463.
[2]. Jenness DD, Burkholder AC, Hartwell LH. Binding of α-factor pheromone to yeast a cells: chemical and genetic evidence for an α-factor receptor. Cell. 1983 Dec 1; 35(2): 521-529.
[3]. Sieber B, Coronas-Serna JM, Martin SG. A focus on yeast mating: From pheromone signaling to cell-cell fusion. InSeminars in Cell & Developmental Biology 2023 Jan 15; 133: 83-95.
| Cas No. | 59401-28-4 | SDF | |
| Canonical SMILES | Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr | ||
| Formula | C82H114N20O17S | M.Wt | 1684 |
| 溶解度 | Soluble in Water | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 593.8 μL | 2.9691 mL | 5.9382 mL |
| 5 mM | 118.8 μL | 593.8 μL | 1.1876 mL |
| 10 mM | 59.4 μL | 296.9 μL | 593.8 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)