α-Factor Mating Pheromone, yeast (Mating Factor α) |
| Catalog No.GC30587 |
α-Factor Mating Pheromone, yeast (Mating Factor α)은 13개의 아미노산으로 구성된 펩타이드 호르몬으로Saccharomyces cerevisiae의 MATα 유형 세포에서 지속적으로 합성되고 분비되고 MATa 유형 세포에 구체적으로 작용할 수 있다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 59401-28-4
Sample solution is provided at 25 µL, 10mM.
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α-Factor Mating Pheromone, yeast (Mating Factor α)은 13개의 아미노산으로 구성된 펩타이드 호르몬으로Saccharomyces cerevisiae의 MATα 유형 세포에서 지속적으로 합성되고 분비되고 MATa 유형 세포에 구체적으로 작용할 수 있다. α-Factor Mating Pheromone은 MATa 세포 표면의 G 단백질 결합 수용체 Ste2p에 결합하고 세포주기 정지, 형태 변화 및 교배 관련 유전자 표현을 유발한다. 이 인자는 방사성 라벨링된 ³⁵S-α 팩터와 결합을 경쟁할 수 있다 (Kd = 0.3 µM). α-Factor Mating Pheromone과 열감수성 ste2 돌연변이체의 결합은 열적으로 불안정하지만 다른 돌연변이체 (ste4, ste5, ste7, ste11 및 ste12)와의 결합은 열적으로 불안정하지 않는다[2]. α-Factor Mating Pheromone은 일반적으로 효모를 교배 상태로 유도할 때 사용되고 외래 단백질의 분비와 표현에 일반적으로 사용된다[3].
References:
[1]. Naider F, Becker JM. The α-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors. Peptides. 2004 Sep 1; 25(9): 1441-1463.
[2]. Jenness DD, Burkholder AC, Hartwell LH. Binding of α-factor pheromone to yeast a cells: chemical and genetic evidence for an α-factor receptor. Cell. 1983 Dec 1; 35(2): 521-529.
[3]. Sieber B, Coronas-Serna JM, Martin SG. A focus on yeast mating: From pheromone signaling to cell-cell fusion. InSeminars in Cell & Developmental Biology 2023 Jan 15; 133: 83-95.
| Cas No. | 59401-28-4 | SDF | |
| Canonical SMILES | Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr | ||
| Formula | C82H114N20O17S | M.Wt | 1684 |
| Solubility | Soluble in Water | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 593.8 μL | 2.9691 mL | 5.9382 mL |
| 5 mM | 118.8 μL | 593.8 μL | 1.1876 mL |
| 10 mM | 59.4 μL | 296.9 μL | 593.8 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
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