AVE5688 |
カタログ番号GC31627
One-Click Copy Product Info
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AVE5688 はグリコーゲンホスホリラーゼ (GP) の阻害剤であり、IC50 は 430 nM と 915 nM、Kds はウサギ筋グリコーゲンホスホリラーゼ (それぞれ rmGPb と rmGPa) に対して 170 nM と 530 nM です。 AVE5688 は 2 型糖尿病の研究に使用できます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 613260-13-2
Sample solution is provided at 25 µL, 10mM.
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AVE5688 is an inhibitor of glycogen phosphorylase (GP), with IC50s of 430 nM and 915 nM and Kds of 170 nM and 530 nM for rabbit muscle glycogen phosphorylase (rmGPb and rmGPa, respectively); AVE5688 can be used for the research of type 2 diabetes.
AVE5688 is an inhibitor of glycogen phosphorylase (GP), with IC50s of 430 nM and 915 nM and Kds of 170 nM and 530 nM for rabbit muscle glycogen phosphorylase (rmGPb and rmGPa, respectively)[1].
[1]. Anderka O, et al. Thermodynamic characterization of allosteric glycogen phosphorylase inhibitors. Biochemistry. 2008 Apr 22;47(16):4683-91.
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Kinase experiment: |
Glycogen phosphorylase activity is measured in the physiological direction of glycogen breakdown. rmGP is used for the kinetic characterization of the inhibitors (AVE5688). To routinely check the phosphorylation grade of enzyme batches, activity is measured in the direction of glycogen synthesis; the phosphorylation grade is estimated from the ratio of the activity in the presence of 0.5 mM caffeine to the activity in the presence of 2 mM AMP[1]. |
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References: [1]. Anderka O, et al. Thermodynamic characterization of allosteric glycogen phosphorylase inhibitors. Biochemistry. 2008 Apr 22;47(16):4683-91. |
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| Cas No. | 613260-13-2 | SDF | |
| Canonical SMILES | O=C(O)C1=CC=C(NC(NC(C2=CC(F)=C(F)C=C2Cl)=O)=O)C(OC(F)(F)F)=C1 | ||
| Formula | C16H8ClF5N2O5 | M.Wt | 438.69 |
| 溶解度 | DMSO : 100 mg/mL (227.95 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2795 mL | 11.3976 mL | 22.7951 mL |
| 5 mM | 455.9 μL | 2.2795 mL | 4.559 mL |
| 10 mM | 228 μL | 1.1398 mL | 2.2795 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 36 reference(s) in Google Scholar.)