FIN56

Name: FIN56
Brand: GlpBio
SKU: GC30039
Availability: InStock
Rating: 5 (15 reviews)

カタログ番号GC30039

FIN56は、新規のフェロプターシス誘導物質であり、GPX4の分解を増加させることでフェロプターシスを誘発する。

Products are for research use only. Not for human use. We do not sell to patients.

FIN56 化学構造

Cas No.: 1083162-61-1

サイズ

価格

在庫数

個数

10mM (in 1mL DMSO)	$52.00	在庫あり
5mg	$46.00	在庫あり
10mg	$77.00	在庫あり
25mg	$168.00	在庫あり
50mg	$301.00	在庫あり
100mg	$539.00	在庫あり

Tel：(909) 407-4943 Email: sales@glpbio.com

顧客レビュー

カスタマーレビューに基づきます。

Sample solution is provided at 25 µL, 10mM.

Product has been cited by 1 publications

Anal Chem (2023).PMID:37942555

Description Protocol Chemical Properties Quality Control Product Documents

Related Biological Data

Confocal fluorescence and ratiometric images of A549 cells pretreated with (A) 10 μM FIN56 for 0, 0.5, and 4.5 h, (B) Control and (C)10 μM FIN56 for 24 h, then incubated with 5 μM PPAC-C4 for 30 min.
The ferroptosis inducer FIN56 (10mM in DMSO) was purchased from GLPBIO.
Analytical Chemistry, 2023. PMID: 37942555 IF: 7.4001

Product Documents

Quality Control & SDS

View current batch:

Purity: >98.00%

Protocol

Cell experiment [1]:
Cell lines	LN229 and U118 GBM cell lines
Preparation Method	Cells were plated into 96-well plates and incubated overnight. Different doses of FIN56 (0, 0.1, 0.5, 1.0, 2.0 4.0 and 8.0 µM) was added to wells. 24 h later, CCK-8 solution was added to each well. 2 h later, samples were measured at 450 nm on a microplate reader.
Reaction Conditions	FIN56 (0-8.0 µM);24 h
Applications	FIN56 decreased the cell viability of LN229 and U118 cells in a dose-dependent manner.
Animal experiment [2]:
Animal models	Nude mouse model
Preparation Method	LN229 cells were injected subcutaneously into the right shoulder of 4-week-old nude mice. 2 weeks later, nude mice (n = 10) were divided into two groups, control group and FIN56 treatment group. Subcutaneous tumors were harvested 30 days after treatment.
Dosage form	30 days
Applications	FIN56 decreased tumor volume obviously, FIN56 significantly increased protein levels of 4-HNE.
References: [1]. Zhang X, Guo Y, et,al. FIN56, a novel ferroptosis inducer, triggers lysosomal membrane permeabilization in a TFEB-dependent manner in glioblastoma. J Cancer. 2021 Sep 13;12(22):6610-6619. doi: 10.7150/jca.58500. PMID: 34659551; PMCID: PMC8517990.

FIN56は、新規のフェロプターシス誘導物質であり、GPX4の分解を増加させることでフェロプターシスを誘発する。FIN56はまた、コレステロール合成に関与する酵素であるスクアレン合成酵素を活性化する。

FIN56（0～8.0μM、24時間）はLN229およびU118細胞の細胞生存率を用量依存的に低下させた。FIN56処理後、LN229とU118の細胞周期はGO/G1期で停止した。FIN56（1-5μM、3-24時間）は、膀胱がん（BC）細胞においてオートファジーに関連した細胞死を誘導する。FIN56（0-20μM）によるGpx4の阻害は、HG誘発フェロプトーシスに対するNACの保護効果を消失させた。サルシンβのタンパク質発現は、FIN56のようなフェロプトーシス活性化因子によってアップレギュレートされた。フェロスタチン1（フェロプシス阻害剤）で前処理すると、HGに暴露したHK 2細胞におけるサルシンβのタンパク質の発現上昇が抑制された。AS-IVは著しく増殖を促進し、アポトーシスを抑制し、活性酸素とLDHの蓄積を減少させた。SCIに対するAS-IVの効果はsi-TFEBによって阻害され、この阻害はFIN56(5μM)の添加によってさらに強化された。

FIN56（30日間）は腫瘍体積を明らかに減少させ、FIN56は4-HNEのタンパク質レベルを有意に増加させた。

References:
[1]. Shimada K, Skouta R, et,al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol. 2016 Jul;12(7):497-503. doi: 10.1038/nchembio.2079. Epub 2016 May 9. PMID: 27159577; PMCID: PMC4920070.
[2]. Gaschler MM, Andia AA, et,al.FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 2018 May;14(5):507-515. doi: 10.1038/s41589-018-0031-6. Epub 2018 Apr 2. PMID: 29610484; PMCID: PMC5899674.
[3]. Zhang X, Guo Y, et,al. FIN56, a novel ferroptosis inducer, triggers lysosomal membrane permeabilization in a TFEB-dependent manner in glioblastoma. J Cancer. 2021 Sep 13;12(22):6610-6619. doi: 10.7150/jca.58500. PMID: 34659551; PMCID: PMC8517990.
[4]. Lei P, Bai T, et,al.Mechanisms of Ferroptosis and Relations With Regulated Cell Death: A Review. Front Physiol. 2019 Feb 26;10:139. doi: 10.3389/fphys.2019.00139. PMID: 30863316; PMCID: PMC6399426.
[5]. Li Q, Liao J,et,al. NAC alleviative ferroptosis in diabetic nephropathy via maintaining mitochondrial redox homeostasis through activating SIRT3-SOD2/Gpx4 pathway. Free Radic Biol Med. 2022 Jul;187:158-170. doi: 10.1016/j.freeradbiomed.2022.05.024. Epub 2022 May 31. Erratum in: Free Radic Biol Med. 2022 Aug 20;189:1. PMID: 35660452.
[6].Wang WJ, Jiang X,et,al.Salusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner. Mol Med Rep. 2021 Sep;24(3):674. doi: 10.3892/mmr.2021.12313. Epub 2021 Jul 23. PMID: 34296310; PMCID: PMC8335735.
[7]. Zhou Y, Li L, et,al.Astragaloside IV ameliorates spinal cord injury through controlling ferroptosis in H2O2-damaged PC12 cells in vitro. Ann Transl Med. 2022 Nov;10(21):1176. doi: 10.21037/atm-22-5196. PMID: 36467371; PMCID: PMC9708485.

Cas No.	1083162-61-1	SDF
Canonical SMILES	O=S(C1=CC(/C2=N\O)=C(C3=C2C=C(S(=O)(NC4CCCCC4)=O)C=C3)C=C1)(NC5CCCCC5)=O
Formula	C₂₅H₃₁N₃O₅S₂	M.Wt	517.66
溶解度	DMSO : ≥ 100 mg/mL (193.18 mM);Water : < 0.1 mg/mL (insoluble)	Storage	Store at -20°C
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
		1 mg	5 mg	10 mg
	1 mM	1.9318 mL	9.6588 mL	19.3177 mL
	5 mM	0.3864 mL	1.9318 mL	3.8635 mL
	10 mM	0.1932 mL	0.9659 mL	1.9318 mL

モルアリティ計算機
希釈計算機

計算

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal:μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

レビュー

Review for FIN56

Average Rating: 5 ★★★★★ (Based on Reviews and 15 reference(s) in Google Scholar.)

5 Star

100%

4 Star

3 Star

2 Star

1 Star

Review for FIN56

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

Overall Rating

★

Review Title

レビュー

ニックネーム

Eメール

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

FIN56

顧客レビュー

GlpBio Citations

Bioactive Compounds Premium Provider

Product has been cited by 1 publications

Related Biological Data

Product Documents

Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

モルアリティ計算機

希釈計算機

In vivo Formulation Calculator (Clear solution)

レビュー

FIN56

顧客レビュー

GlpBio Citations

Bioactive Compounds Premium Provider

Product has been cited by 1 publications

Related Biological Data

Product Documents

Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

モルアリティ計算機

希釈計算機

In vivo Formulation Calculator (Clear solution)

Related Products

レビュー