Diphenyl Blue (Direct Blue 14) |
カタログ番号GC30152 |
ジフェニル ブルー (Direct Blue 14) (Direct Blue 14) は、アゾ染料のグループの参照染料として使用されます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 72-57-1
Sample solution is provided at 25 µL, 10mM.
Diphenyl Blue (Trypan blue), a cell viability dye, is the most commonly used dye to identify dead cells and is used to test cell membrane integrity and cell viability[1]. Normal living cells have a complete membrane structure, which can repel trypan blue and prevent it from entering the cell; while cells with inactive or incomplete membranes have increased membrane permeability and can be stained blue by trypan blue It is generally considered that the integrity of the cell membrane is lost, that is, the cell is considered to be dead, which is opposite to the effect of neutral red. Therefore, with the help of trypan blue staining, it is very simple and fast to distinguish living cells from dead cells. Trypan blue is one of the most commonly used stains for dead cell identification in tissue and cell culture[1]. Macrophages are able to phagocytose diphenyl blue, so it can be used as a live stain for macrophages[2].
References:
[1]. B A Avelar-Freitas,et. Trypan blue exclusion assay by flow cytometry. 2014 Apr;47(4):307-15. doi: 10.1590/1414-431X20143437. Epub 2014 Mar 18.
[2]. Daly, M. L., DeRosa, C. A., Kerr, C., Morris, W. A., & Fraser, C. L. (2016). Blue thermally activated delayed fluorescence from a biphenyl difluoroboron β-diketonate. RSC Advances, 6(85), 81631–81635. doi:10.1039/c6ra18374c.
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