13,14-dihydro-15-keto Prostaglandin E2-d4 MaxSpec® Standard (Synonyms: 13,14-dh-15-keto PGE2-d4, 13,14-dihydro-oxo-PGE2-d4, PGEM-d4)

13,14-dihydro-15-keto Prostaglandin E2-d4 (13,14-dh-15-keto PGE2-d4) is intended for use as an internal standard for the quantification of 13,14-dh-15-keto PGE2 by GC- or LC-MS. 13,14-dh-15-keto PGE2 is the primary metabolite of PGE2 in plasma. Endogenous or infused PGE2 is rapidly metabolized by the enzymes 15-hydroxy PGDH and 15-oxo-PG δ13-reductase to form 13,14-dh-15-keto PGE2. 13,14-dh-15-keto PGE2 accumulates to detectable levels; plasma levels in humans are between 10-100 pg/ml. It undergoes further metabolism and chemical decomposition, giving it a relatively short half-life. In dogs, the plasma half-life of 13,14-dh-15-keto PGE2 is about 9 minutes. In humans the metabolite has a similar short half-life, making it a poor choice of analytes for assays designed to measure total PGE2 biosynthesis. 13,14-dh-15-keto PGE2-d4 MaxSpec® standard is a quantitative grade standard of 13,14-dh-15-keto PGE2-d4 that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This 13,14-dh-15-keto PGE2-d4 MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.