2,4-Dinitrobenzenesulfonic acid hydrate |
カタログ番号GD08485
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2,4-Dinitrobenzenesulfonic acid hydrateはベンゼンスルホン酸の誘導体であり、体で様々な炎症を引き起こし、よく大腸炎モデルなどの動物モデルを建てる誘導剤として使われている。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 698999-22-3
Sample solution is provided at 25 µL, 10mM.
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2,4-Dinitrobenzenesulfonic acid hydrateはベンゼンスルホン酸の誘導体であり、体で様々な炎症を引き起こし[1-2]、よく大腸炎モデルなどの動物モデルを建てる誘導剤として使われている[3]。
2,4-Dinitrobenzenesulfonic acidは、ジオールの関与するアミノ化反応の触媒剤として使われ、相応のα-ヒドロキシケトンの合成にも使われることが可能である[4]。
体内で、重症の大腸炎マウスモデルを建てるために、マウスを麻酔し、長さ10cmのPE-90カテーテルを結腸の3.5cmに挿入した。用量が200mg/kgの2,4-Dinitrobenzenesulfonic acidの溶液をカテーテルを通じて注射した。12日目に、100mg/kgの2,4-Dinitrobenzenesulfonic acid溶液の二回目の注射で、大腸炎を再び活性化した。軽度の腸炎モデルで、同じ手続きでカテーテルを置き、最初の注射の21日後に、100mg/kgの2,4-Dinitrobenzenesulfonic acidの二回目の注射で、腸炎を再び活性化した[5]。慢性腸炎のモデルを建てるために、マウスを麻酔した後、長さ10cmのPE-90カテーテルを、マウスの結腸の3.5cmに挿入し、2,4-Dinitrobenzenesulfonic acid溶液を、6週間連続、毎週2mg, 2mg, 4mg, 4mg, 6mg, 6mgと6mgの注射用量でカテーテルを通じて注射した[6]。急性内臓痛モデルを建てるため、短い間に2%のイソフルラン麻酔の後、PE-60カテーテルを、肛門から8cm離れた直腸に挿入し、0.25mlの50%エタノールに溶解された30mgの2,4-Dinitrobenzenesulfonic acidを、14から21日間、カテーテルを通じて体に注射した[7]。前立腺炎モデルを建てるため、マウスを麻酔し、エチレンオキシドで殺菌され、潤滑されたPE10カテーテルを尿道の1.5cmに挿入した。体積が40μlの10mg/ml 2,4-Dinitrobenzenesulfonic acid溶液を注射し、誘発の2日後に、症状が一番顕著になった[8]。
References:
[1] Kirby J, Standfest M, Binkley J, et al. The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification. J Appl Oral Sci. 2024 Sep 30;32:e20240224.
[2] Kim S, Lee S, Lee H, et al, A Colon-Targeted Prodrug, 4-Phenylbutyric Acid-Glutamic Acid Conjugate, Ameliorates 2,4-Dinitrobenzenesulfonic Acid-Induced Colitis in Rats. Pharmaceutics. 2020 Sep 3;12(9):843.
[3] Morampudi V, Bhinder G, Wu X, et al. DNBS/TNBS colitis models: providing insights into inflammatory bowel disease and effects of dietary fat. J Vis Exp. 2014 Feb 27;(84):e51297.
[4] Karmakar P, Talan RS, Sucheck SJ. Mixed-phase synthesis of glycopeptides using a N-peptidyl-2,4-dinitrobenzenesulfonamide-thioacid ligation strategy. Org Lett. 2011 Oct 7;13(19):5298-301.
[5] Martín R, Chain F, Miquel S, et al. Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles. Immune Netw. 2024 Dec 24;24(6):e44.
[6] Choi AR, Jung MJ, Kim JH, et al. Co-treatment of Salinomycin Sensitizes AZD5363-treated Cancer Cells Through Increased Apoptosis. Anticancer Res. 2015 Sep;35(9):4741-7.
[7] Lucarini E, Coppi E, Micheli L, et al. Acute visceral pain relief mediated by A3AR agonists in rats: involvement of N-type voltage-gated calcium channels. Pain. 2020 Sep 1;161(9):2179-2190.
[8] Stanton MM, Nelson LK, Benediktsson H, et al. Proteinase-activated receptor-1 and immunomodulatory effects of a PAR1-activating peptide in a mouse model of prostatitis. Mediators Inflamm. 2013;2013:748395.
重症大腸炎モデル
1.動物の麻酔：
マウスを2%のイソフルランで麻酔した。
2.モデル確立：長さ10cmのPE-90カテーテルを結腸の3.5cmに挿入した。200mg/kgの2,4 - dinitrobenzenesulfonic acid (30%のエタノールに溶解)溶液をカテーテルを通じて注射した。マウスの対照群には30%のエタノール処理のみを行った。DNBS注射の最初の3日間で、マウスに、6%の蔗糖を含有する水を飲ませ、脱水を予防した。
3.大腸炎の再活性化：最初の2,4 - dinitrobenzenesulfonic acid注射の4日間の回復期の後、マウスをいくつかのグループに分け、特定の細菌株またはその培養上清を7日間経口投与した。最初のDNBS注射後の12日目に、二回目の100mg/kg DNBS溶液の注射で大腸炎を再活性化した。
References:
[1] Martín R, Chain F, Miquel S, et al. Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles. Immune Netw. 2024 Dec 24;24(6):e44.
| Cas No. | 698999-22-3 | SDF | |
| Formula | C6H6N2O8S | M.Wt | 266.19 |
| 溶解度 | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7567 mL | 18.7836 mL | 37.5672 mL |
| 5 mM | 751.3 μL | 3.7567 mL | 7.5134 mL |
| 10 mM | 375.7 μL | 1.8784 mL | 3.7567 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)