2,4-Dinitrobenzenesulfonic acid hydrate |
Katalog-Nr.GD08485
One-Click Copy Product Info
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2,4-Dinitrobenzenesulfonic acid hydrateis a derivative of benzenesulfonic acid, which can cause various inflammations in the body, and is commonly used as an inducer to establish animal models, such as colitis models.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 698999-22-3
Sample solution is provided at 25 µL, 10mM.
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2,4-Dinitrobenzenesulfonic acid hydrate is a derivative of benzenesulfonic acid, which can cause various inflammations in the body[1-2], and is commonly used as an inducer to establish animal models, such as colitis models[3]. 2,4-Dinitrobenzenesulfonic acid can be used as a catalyst for an amination reaction involving diols, and can also be used to synthesize the corresponding α-hydroxyketone[4].
In vivo, to establish a severe colitis mouse model, mice are anesthetized, and a 10-cm-long PE-90 catheter is inserted 3.5cm into the colon. A solution of 2,4-Dinitrobenzenesulfonic acid at a dose of 200mg/kg is injected through the catheter. On day 12, colitis is reactivated by a second injection of a 100mg/kg 2,4-Dinitrobenzenesulfonic acid solution. In the mild enteritis model, the catheter is placed using the same procedure, and the enteritis is reactivated by a second injection of 100mg/kg of 2,4-Dinitrobenzenesulfonic acid solution on day 21 after the first injection of 2,4-Dinitrobenzenesulfonic acid[5]. In order to establish a model of chronic enteritis, after anesthetizing the mice, a 10cm long PE-90 catheter was inserted into the colon of the mice at 3.5cm, and 2,4-Dinitrobenzenesulfonic acid solution was injected through the catheter at weekly injection doses of 2mg, 2mg, 4mg, 4mg, 6mg, 6mg, and 6mg for six consecutive weeks[6]. To establish a model of acute visceral pain, after a brief 2% isoflurane anesthesia, a PE-60 catheter was inserted into the rectum 8cm away from the anus, and 30mg of 2,4-Dinitrobenzenesulfonic acid dissolved in 0.25ml of 50% ethanol was injected into the body through the catheter for 14 to 21 days[7]. To establish a prostatitis model, mice are anesthetized, and a PE10 catheter sterilized with ethylene oxide and lubricated is inserted 1.5cm into the urethra. A volume of 40μl of a 10mg/ml 2,4-Dinitrobenzenesulfonic acid solution is injected, with symptoms being most pronounced on the second day after induction[8].
References:
[1] Kirby J, Standfest M, Binkley J, et al. The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification. J Appl Oral Sci. 2024 Sep 30;32:e20240224.
[2] Kim S, Lee S, Lee H, et al, A Colon-Targeted Prodrug, 4-Phenylbutyric Acid-Glutamic Acid Conjugate, Ameliorates 2,4-Dinitrobenzenesulfonic Acid-Induced Colitis in Rats. Pharmaceutics. 2020 Sep 3;12(9):843.
[3] Morampudi V, Bhinder G, Wu X, et al. DNBS/TNBS colitis models: providing insights into inflammatory bowel disease and effects of dietary fat. J Vis Exp. 2014 Feb 27;(84):e51297.
[4] Karmakar P, Talan RS, Sucheck SJ. Mixed-phase synthesis of glycopeptides using a N-peptidyl-2,4-dinitrobenzenesulfonamide-thioacid ligation strategy. Org Lett. 2011 Oct 7;13(19):5298-301.
[5] Martín R, Chain F, Miquel S, et al. Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles. Immune Netw. 2024 Dec 24;24(6):e44.
[6] Choi AR, Jung MJ, Kim JH, et al. Co-treatment of Salinomycin Sensitizes AZD5363-treated Cancer Cells Through Increased Apoptosis. Anticancer Res. 2015 Sep;35(9):4741-7.
[7] Lucarini E, Coppi E, Micheli L, et al. Acute visceral pain relief mediated by A3AR agonists in rats: involvement of N-type voltage-gated calcium channels. Pain. 2020 Sep 1;161(9):2179-2190.
[8] Stanton MM, Nelson LK, Benediktsson H, et al. Proteinase-activated receptor-1 and immunomodulatory effects of a PAR1-activating peptide in a mouse model of prostatitis. Mediators Inflamm. 2013;2013:748395.
Severe colitis model
1.Animal anesthesia:
Mice were anesthetized with 2% isoflurane.
2.Model establishment: A 10-cm-long PE-90 catheter was inserted 3.5cm into the colon. A solution of 200mg/kg 2,4 - dinitrobenzenesulfonic acid (dissolved in 30% ethanol) was injected through the catheter. The control group of mice received only 30% ethanol treatment. For the first 3 days after DNBS injection, the mice drank water containing 6% sucrose to prevent dehydration.
3.Reactivation of colitis: After a 4-day recovery period following the initial 2,4 - dinitrobenzenesulfonic acid injection, the mice were divided into several groups and were given specific bacterial strains or their culture supernatants via gavage for 7 days. On day 12 after the first DNBS injection, colitis was reactivated by a second injection of 100mg/kg DNBS solution.
References:
[1] Martín R, Chain F, Miquel S, et al. Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles. Immune Netw. 2024 Dec 24;24(6):e44.
| Cas No. | 698999-22-3 | SDF | |
| Formula | C6H6N2O8S | M.Wt | 266.19 |
| Löslichkeit | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.7567 mL | 18.7836 mL | 37.5672 mL |
| 5 mM | 751.3 μL | 3.7567 mL | 7.5134 mL |
| 10 mM | 375.7 μL | 1.8784 mL | 3.7567 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)