L-Ascorbic Acid 2-phosphate (magnesium salt) (Synonyms: AA2P, Ascorbyl PM, Phospitan C) |
カタログ番号GC19511
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L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)(AA2P)は、安定したVC(ビタミンC)誘導体である。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 113170-55-1
Sample solution is provided at 25 µL, 10mM.
L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)(AA2P)は、安定したVC(ビタミンC)誘導体である[1]。コラーゲンの発現と形成を刺激することができる[2]。
L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)(100-500μM;24時間)は、SH-SY5Y細胞のROS媒介性DNA損傷を緩和することにより、MeHg誘導性アポトーシスを弱める[3]。L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)(0.25-1mM)は、ジヒドロテストステロン(DHT)誘導性DHT誘導性ジッコフ-1(DKK-1)タンパク質発現を抑制した[4]。L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)(250μM;5-60分)とFGF-2の同時処理は、ヒト骨髄間葉系幹細胞(BMSC)の最適な増殖を誘導し、2ヶ月間の培養期間中にBMSC数の蓄積率を増加させた[5]。毛乳頭(DP)細胞のIGF-1 mRNAはアップレギュレーションされ、DP細胞の馴化培地中のIGF-1タンパク質は、L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)、L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)-DP細胞からの誘導性IGF-1で処理した後、濾胞性ケラチノサイトの増殖を促進し、PI3Kを介してin vitroで毛包の成長を刺激する[6]。
L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)は、マウスモデルにおいて、毛包の間葉系-上皮間相互作用を調節し、毛髪の成長を促進する[7]。
References:
[1]. Spiclin P, Homar M, et,al. Sodium ascorbyl phosphate in topical microemulsions. Int J Pharm. 2003 Apr 30;256(1-2):65-73. doi: 10.1016/s0378-5173(03)00063-2. PMID: 12695012.
[2]. Shima N, Kimoto M, et,al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate. Invest Ophthalmol Vis Sci. 2011 Nov 7;52(12):8711-7. doi: 10.1167/iovs.11-7592. PMID: 21980003.
[3]. Zuo K, Xu Q, et,al. L-Ascorbic Acid 2-Phosphate Attenuates Methylmercury-Induced Apoptosis by Inhibiting Reactive Oxygen Species Accumulation and DNA Damage in Human SH-SY5Y Cells. Toxics. 2023 Feb 2;11(2):144. doi: 10.3390/toxics11020144. PMID: 36851019; PMCID: PMC9967424.
[4]. Kwack MH, Kim MK, et,al. L-ascorbic acid 2-phosphate represses the dihydrotestosterone-induced dickkopf-1 expression in human balding dermal papilla cells. Exp Dermatol. 2010 Dec;19(12):1110-2. doi: 10.1111/j.1600-0625.2010.01143.x. PMID: 20701628.
[5]. Bae SH, Ryu H, et,al. L-ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. Growth Factors. 2015 Apr;33(2):71-8. doi: 10.3109/08977194.2015.1013628. Epub 2015 Feb 25. PMID: 25714612.
[6]. Kwack MH, Shin SH, et,al. l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase. Br J Dermatol. 2009 Jun;160(6):1157-62. doi: 10.1111/j.1365-2133.2009.09108.x. Epub 2009 Mar 26. PMID: 19416266.
[7]. Sung YK, Hwang SY, et,al. The hair growth promoting effect of ascorbic acid 2-phosphate, a long-acting Vitamin C derivative. J Dermatol Sci. 2006 Feb;41(2):150-2. doi: 10.1016/j.jdermsci.2005.11.010. Epub 2006 Jan 9. PMID: 16406749.
| 細胞実験[1]: | |
細胞株 | SH-SY5Y細胞 |
準備方法 | SH-SY5Y細胞は、まず100-500 μM L-ASCORBIC ACID 2-PHOSPHATE含有1×PBSで調製し、1 μM MeHgで24時間処理した。 |
反応条件 | 100-500μM;24時間 |
アプリケーション | L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)は、細胞内のMeHg誘導性ROS蓄積を用量依存的に減少させる。 |
| 動物実験 [2]: | |
動物モデル | C57BL/6マウス |
準備方法 | マウスの背部皮膚を7週齢(休止期)に剃毛し、次いでビヒクルまたはL-ASCORBIC ACID 2-PHOSPHATE2-リン酸を250mMでイオントフォレーシスにより適用した。独自のイオントフォレーシスシステムを採用し、0.1-1.0mA/cm2領域の一定の電流を供給する。ロントフォレーシスは、0.2〜0.4mVの定電流で毎日15分間、4週間にわたって実施した。 |
投与形態 | |
アプリケーション | L-ASCORBIC ACID 2-PHOSPHATE (MAGNESIUM SALT)は、マウスモデルにおいて、毛包における間葉系-上皮間相互作用を調節し、発毛を促進する。 |
参考文献: [1]. Zuo K, Xu Q, et,al. L-Ascorbic Acid 2-Phosphate Attenuates Methylmercury-Induced Apoptosis by Inhibiting Reactive Oxygen Species Accumulation and DNA Damage in Human SH-SY5Y Cells. Toxics. 2023 Feb 2;11(2):144. doi: 10.3390/toxics11020144. PMID: 36851019; PMCID: PMC9967424. [2]. Sung YK, Hwang SY, et,al. The hair growth promoting effect of ascorbic acid 2-phosphate, a long-acting Vitamin C derivative. J Dermatol Sci. 2006 Feb;41(2):150-2. doi: 10.1016/j.jdermsci.2005.11.010. Epub 2006 Jan 9. PMID: 16406749. | |
| Cas No. | 113170-55-1 | SDF | |
| 同義語 | AA2P, Ascorbyl PM, Phospitan C | ||
| Chemical Name | L-Ascorbic acid is essential for the synthesis of collagen | ||
| Canonical SMILES | O=C1C(OP([O-])([O-])=O)=C([C@]([H])(O1)[C@H](CO)O)[O-].O=C2C(OP([O-])([O-])=O)=C([C@]([H])(O2)[C@H](CO)O)[O-].[Mg+2].[Mg+2].[Mg+2] GC47538: O=C1C(OP([O-])([O-])=O)=C([C@]([H])(O1)[C@H](CO)O)[O-].O=C2C(OP([O-])([O-])=O)=C([C@]([H])(O2)[C@H](CO)O)[O-].[Mg+2].[Mg+2].[Mg+2] | ||
| Formula | C6H6O9P • 1.5Mg | M.Wt | 289.5 |
| 溶解度 | Water:25mg/ml | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4542 mL | 17.2712 mL | 34.5423 mL |
| 5 mM | 690.8 μL | 3.4542 mL | 6.9085 mL |
| 10 mM | 345.4 μL | 1.7271 mL | 3.4542 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
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Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)