L-Ascorbic Acid 2-phosphate (magnesium salt) (Synonyms: AA2P, Ascorbyl PM, Phospitan C) |
Catalog No.GC19511
One-Click Copy Product Info
|
L-Ascorbic Acid 2-phosphate (magnesium salt) (AA2P) is a stable VC(vitamin C) derivative.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 113170-55-1
Sample solution is provided at 25 µL, 10mM.
L-Ascorbic Acid 2-phosphate (magnesium salt) (AA2P) is a stable VC(vitamin C) derivative[1]. It can stimulate the expression and formation of collagen[2].
L-Ascorbic Acid 2-phosphate (magnesium salt) (100-500μM; 24 h) attenuates MeHg-induced apoptosis by alleviating ROS-mediated DNA damage in SH-SY5Y cell [3]. L-Ascorbic Acid 2-phosphate (magnesium salt) (0.25-1mM) repressed dihydrotestosterone (DHT)-induced DHT-inducible dickkopf-1 (DKK-1) protein expression[4]. L-Ascorbic Acid 2-phosphate (magnesium salt) (250μM; 5-60min) co-treatment with FGF-2 induced optimal proliferation of Human bone marrow mesenchymal stem cells (BMSC) and increased the accumulation rate of BMSC numbers during a 2-month culture period[5]. IGF-1 mRNA in Dermal papilla (DP) cells was upregulated and IGF-1 protein in the conditioned medium of DP cells was significantly increased after treatment with L-Ascorbic Acid 2-phosphate (magnesium salt), L-Ascorbic Acid 2-phosphate (magnesium salt)-inducible IGF-1 from DP cells promotes proliferation of follicular keratinocytes and stimulates hair follicle growth in vitro via PI3K[6].
L-Ascorbic Acid 2-phosphate (magnesium salt) regulate mesenchymal–epithelial interactions in the hair follicle and promote hair growth in mice models[7].
References:
[1]. Spiclin P, Homar M, et,al. Sodium ascorbyl phosphate in topical microemulsions. Int J Pharm. 2003 Apr 30;256(1-2):65-73. doi: 10.1016/s0378-5173(03)00063-2. PMID: 12695012.
[2]. Shima N, Kimoto M, et,al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate. Invest Ophthalmol Vis Sci. 2011 Nov 7;52(12):8711-7. doi: 10.1167/iovs.11-7592. PMID: 21980003.
[3]. Zuo K, Xu Q, et,al. L-Ascorbic Acid 2-Phosphate Attenuates Methylmercury-Induced Apoptosis by Inhibiting Reactive Oxygen Species Accumulation and DNA Damage in Human SH-SY5Y Cells. Toxics. 2023 Feb 2;11(2):144. doi: 10.3390/toxics11020144. PMID: 36851019; PMCID: PMC9967424.
[4]. Kwack MH, Kim MK, et,al. L-ascorbic acid 2-phosphate represses the dihydrotestosterone-induced dickkopf-1 expression in human balding dermal papilla cells. Exp Dermatol. 2010 Dec;19(12):1110-2. doi: 10.1111/j.1600-0625.2010.01143.x. PMID: 20701628.
[5]. Bae SH, Ryu H, et,al. L-ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. Growth Factors. 2015 Apr;33(2):71-8. doi: 10.3109/08977194.2015.1013628. Epub 2015 Feb 25. PMID: 25714612.
[6]. Kwack MH, Shin SH, et,al. l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase. Br J Dermatol. 2009 Jun;160(6):1157-62. doi: 10.1111/j.1365-2133.2009.09108.x. Epub 2009 Mar 26. PMID: 19416266.
[7]. Sung YK, Hwang SY, et,al. The hair growth promoting effect of ascorbic acid 2-phosphate, a long-acting Vitamin C derivative. J Dermatol Sci. 2006 Feb;41(2):150-2. doi: 10.1016/j.jdermsci.2005.11.010. Epub 2006 Jan 9. PMID: 16406749.
|
Cell experiment [1]: |
|
|
Cell lines |
SH-SY5Y cells |
|
Preparation method |
SH-SY5Y cells were first prepared with 100-500 μM L-Ascorbic Acid 2-phosphate in 1×PBS and treated with 1 μM MeHg for 24 h. |
|
Reaction Conditions |
100-500μM; 24 h |
|
Applications |
L-Ascorbic Acid 2-phosphate (magnesium salt) decreases MeHg-induced ROS accumulation in cells in a dose-dependent manner. |
|
Animal experiment [2]: |
|
|
Animal models |
C57BL/6 mice |
|
Preparation method |
Mice back skins were shaved at 7 weeks of age (telogen stage) and then vehicle or L-Ascorbic Acid 2-phosphate at 250 mM were applied by iontophoresis. The unique iontophoresis system was adapted, which provides a constant electric current of 0.1-1.0 mA/cm2 areas. Lontophoresis was performed for 15 min at 0.2-0.4 mV constant currents daily for 4 weeks. |
|
Applications |
L-Ascorbic Acid 2-phosphate (magnesium salt) regulate mesenchymal–epithelial interactions in the hair follicle and promote hair growth in mice models. |
|
References: [1]. Zuo K, Xu Q, et,al. L-Ascorbic Acid 2-Phosphate Attenuates Methylmercury-Induced Apoptosis by Inhibiting Reactive Oxygen Species Accumulation and DNA Damage in Human SH-SY5Y Cells. Toxics. 2023 Feb 2;11(2):144. doi: 10.3390/toxics11020144. PMID: 36851019; PMCID: PMC9967424. [2]. Sung YK, Hwang SY, et,al. The hair growth promoting effect of ascorbic acid 2-phosphate, a long-acting Vitamin C derivative. J Dermatol Sci. 2006 Feb;41(2):150-2. doi: 10.1016/j.jdermsci.2005.11.010. Epub 2006 Jan 9. PMID: 16406749. |
|
| Cas No. | 113170-55-1 | SDF | |
| Synonyms | AA2P, Ascorbyl PM, Phospitan C | ||
| Chemical Name | L-Ascorbic acid is essential for the synthesis of collagen | ||
| Canonical SMILES | O=C1C(OP([O-])([O-])=O)=C([C@]([H])(O1)[C@H](CO)O)[O-].O=C2C(OP([O-])([O-])=O)=C([C@]([H])(O2)[C@H](CO)O)[O-].[Mg+2].[Mg+2].[Mg+2] GC47538: O=C1C(OP([O-])([O-])=O)=C([C@]([H])(O1)[C@H](CO)O)[O-].O=C2C(OP([O-])([O-])=O)=C([C@]([H])(O2)[C@H](CO)O)[O-].[Mg+2].[Mg+2].[Mg+2] | ||
| Formula | C6H6O9P • 1.5Mg | M.Wt | 289.5 |
| Solubility | Water:25mg/ml | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.4542 mL | 17.2712 mL | 34.5423 mL |
| 5 mM | 690.8 μL | 3.4542 mL | 6.9085 mL |
| 10 mM | 345.4 μL | 1.7271 mL | 3.4542 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)