Taurodeoxycholic Acid (sodium salt)

Cell lines

The human colonic epithelial cell line HT-29

Preparation Method

The human CEC line HT-29 were treated with dilutions of Taurodeoxycholic acid (TDCA). and IL-8 secretion was analyzed in the supernatant 24 h after stimulation.NF-kappa B binding activity was analyzed with EMSA, RelA translocation with immunofluorescence, and RelA-phosphorylation with Western blot analysis.

Reaction Conditions

1,500 μM Taurodeoxycholic acid for 24h.

Applications

Taurodeoxycholic acid induced IL-8 gene expression correlated with enhanced RelA phosphorylation, which was blocked by Ad5dnIKK beta.Taurodeoxycholic acid primarily induced IL-8 gene expression through RelA phosphorylation.

Animal models

C57Bl/6J  mice

Preparation Method

Mice were fed a liquid diet with or without Taurodeoxycholic acid supplementation. After 6 days, the mice were injected with LPS (10mg/kg) to induce intestinal injury. Specimens were obtained 24 hours later and evaluated for intestinal apoptosis, crypt proliferation, and villus length. A separate cohort of animals were injected with LPS (25mg/kg) and followed 7 days for survival.

Dosage form

50mg/kg/day Taurodeoxycholic acid, oral gavage

Applications

Mice whose diet was supplemented with Taurodeoxycholic acid had significantly increased survival. After LPS-induced injury, mice supplemented with Taurodeoxycholic acid showed decreased intestinal apoptosis by both H&E and caspase-3. Dietary taurodeoxycholic acid supplementation alleviates mucosal damage and improves survival after LPS-induced intestinal injury. Taurodeoxycholic acid is protective of the intestinal mucosa by increasing resistance to injury-induced apoptosis, stimulating enterocyte proliferation and increasing villus length.

References:

[1]. Mühlbauer M, Allard B, et al. Differential effects of deoxycholic acid and taurodeoxycholic acid on NF-kappa B signal transduction and IL-8 gene expression in colonic epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2004 Jun;286(6):G1000-8. 

[2]. Perrone EE, Chen C, et al. Warner BW, Sun CC, Alaish SM, Strauch ED. Dietary bile acid supplementation improves intestinal integrity and survival in a murine model. J Pediatr Surg. 2010 Jun;45(6):1256-65.