7α-hydroxy Cholesterol (Synonyms: 7β-Hydroxycholesterol, 7β-OHC) |
Catalog No.GC49443
One-Click Copy Product Info
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7α-하이드록시 콜레스테롤은 산화 콜레스테롤이며 효소적 산화와 비효소적 산화 모두에 의해 형성됩니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 566-26-7
Sample solution is provided at 25 µL, 10mM.
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7α-hydroxy Cholesterol is an endogenous oxidized cholesterol metabolite. 7α-hydroxy Cholesterol serves as a key rate-limiting intermediate in the bile acid biosynthesis pathway and a potential biomarker for lipid peroxidation. 7α-hydroxy Cholesterol can be used in research related to cardiovascular metabolic diseases such as atherosclerosis, as well as long-acting anti-tumor immunotherapy using TCR-T cells[1-4].
In vitro, 7α-hydroxy Cholesterol (5μg/ml) was applied to treat THP-1 monocytes for 6-48 hours. 7α-hydroxy Cholesterol significantly upregulated the expression of pattern recognition receptors (TLR6 and CD14) and chemokines (CCL2, CCL3, and CCL4). 7α-hydroxy Cholesterol enhanced cellular immune responses to PAMPs (such as FSL-1 and LPS) and immune cell migration, while simultaneously activating the phosphorylation of the Akt, Src, ERK1/2, and NF-κB signaling pathways[5]. 7α-hydroxy Cholesterol (12.5-100ng/ml) was used to treat HeLa cells for 24 hours, followed by stimulation with pyolysin for 2 hours. 7α-hydroxy Cholesterol significantly inhibited lactate dehydrogenase (LDH) leakage and cytolysis. 7α-hydroxy Cholesterol prevented the activation of MAPK cell stress responses and limited alterations in the cytoskeleton[6].
References:
[1] Cho HR, Son Y, Kim SM, et al. 7α-Hydroxycholesterol induces monocyte/macrophage cell expression of interleukin-8 via C5a receptor. PLoS One. 2017 Mar 21;12(3):e0173749.
[2] Crosignani A, Zuin M, Allocca M, et al. Oxysterols in bile acid metabolism. Clin Chim Acta. 2011 Nov 20;412(23-24):2037-45.
[3] Kim BY, Son Y, Kim BJ, et al. Atheroma-Relevant 7-Oxysterols Differentially Upregulate Cd14 Expression. Int J Mol Sci. 2023 Jun 23;24(13):10542.
[4] Nury T, Samadi M, Zarrouk A, et al. Improved synthesis and in vitro evaluation of the cytotoxic profile of oxysterols oxidized at C4 (4α- and 4β-hydroxycholesterol) and C7 (7-ketocholesterol, 7α- and 7β-hydroxycholesterol) on cells of the central nervous system. Eur J Med Chem. 2013;70:558-67.
[5] Son Y, Kim BY, Kim M, et al. Glucocorticoids Impair the 7α-Hydroxycholesterol-Enhanced Innate Immune Response. Immune Netw. 2023 Oct 19;23(5):e40.
[6] Ormsby TJR, Owens SE, Clement L, et al. Oxysterols Protect Epithelial Cells Against Pore-Forming Toxins. Front Immunol. 2022 Jan 26;13:815775.
| Cell experiment [1]: | |
Cell lines | HeLa cells (human epithelial immortal cell line) |
Preparation Method | HeLa cells were maintained in complete DMEM supplemented with 10% fetal bovine serum (FBS) at 37.5°C in humidified air with 5% CO₂. HeLa cells were seeded at 4 x 10⁴ cells/well in 24-well plates and cultured until 70% confluent. Then, cells were treated with 7α-hydroxy Cholesterol (12.5-100ng/ml) in serum-free medium. |
Reaction Conditions | 12.5-100ng/ml; 24h |
Applications | 7α-hydroxy Cholesterol reduced pyolysin-induced lactate dehydrogenase (LDH) leakage and cytolysis. 7α-hydroxy Cholesterol also prevented potassium ion leakage, mitogen-activated protein kinase (MAPK) cell stress responses, and limited alterations in the cytoskeleton. |
References: | |
| Cas No. | 566-26-7 | SDF | |
| Synonyms | 7β-Hydroxycholesterol, 7β-OHC | ||
| Chemical Name | cholest-5-ene-3β,7α-diol | ||
| Canonical SMILES | O[C@H](C1)CC[C@@]2(C)C1=C[C@@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)C | ||
| Formula | C27H46O2 | M.Wt | 402.7 |
| Solubility | DMF: 2 mg/ml, DMSO: 0.1 mg/ml, Ethanol: 20 mg/ml | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4832 mL | 12.4162 mL | 24.8324 mL |
| 5 mM | 496.6 μL | 2.4832 mL | 4.9665 mL |
| 10 mM | 248.3 μL | 1.2416 mL | 2.4832 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)