Baicalin (Synonyms: Baicalein 7-glucuronide) |
Catalog No.GN10018
One-Click Copy Product Info
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Baicalin은 플라보노이드 글리코사이드이고 알로스터릭 카닌 팜리토일전이효소 1 (CPT1) 활성화제이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 21967-41-9
Sample solution is provided at 25 µL, 10mM.
Baicalin은 플라보노이드 글리코사이드이고 알로스터릭 카닌 팜리토일전이효소 1 (CPT1) 활성화제이다. Baicalin은 인류면역결핍 바이러스 유형 1 (HIV-1)의 복제를 억제할 수 있다[2]. Baicalin은 NF-κB 표현을 감소시킬 수 있고 주요 암 신호 전달 경로의 규제를 조절한다[3].
체외 실험에서 Baicalin (0.005-0.5nM) 처리된 RAW264.7 세포는 LPS 유도된 세포 증식을 억제하고 세포 간 adhesion molecules 1 (ICAM-1), monocyte chemoreductant protein 1 (MCP-1), cyclooxygenase 2 (Cox-2) 단백질의 표현을 줄였다[4]. Baicalin (100μM)은 HK-2 세포를 1시간 동안 사전 처리하여 H2O2 자극 후의 세포 생존성을 향상시키고 산화 스트레스를 줄이고 caspase-3 활성화와 세포 사멸을 억제할 수 있다[5].
체내 실험에서 Baicalin (10, 100 mg/kg)은 신장 뇌혈 재관류 정상 (IRI)을 가진 쥐에게 복강 주입으로 투여되었고 이는 산화 스트레스를 감소시키고 조직학적 손상을 줄였고 신장 기능을 개선하고 염증 반응과 튜부 소양을 억제하고 TLR2, TLR4, MyD88, p-NF-κB 및 p-IκB 단백질과 caspase-3 활성성 및 Bcl-2/Bax 비율을 증가시켰다[6]. Baicalin (400 mg/kg)은 당뇨병 신장병 (DN) 쥐에게 복강 주입으로 투여되었고 당뇨병 상태, 단백뇨, 신장 조직 경로학적 변화와 세포 사멸을 효과적으로 개선하고 고전적인 염증 신호 경로인 MAPK 가족의 활성을 억제하는 등 Erk1/2, JNK, P38MAPK 신호 경로[7].
References:
[1] Dai J, Liang K, Zhao S, et al. Chemoproteomics reveals baicalin activates hepatic CPT1 to ameliorate diet-induced obesity and hepatic steatosis[J]. Proceedings of the National Academy of Sciences, 2018, 115(26): E5896-E5905.
[2] Kitamura K, Honda M, Yoshizaki H, et al. Baicalin, an inhibitor of HIV-1 production in vitro[J]. Antiviral research, 1998, 37(2): 131-140.
[3] Yu X, Liu Y, Wang Y, et al. Baicalein induces cervical cancer apoptosis through the NF-κB signaling pathway[J]. Molecular Medicine Reports, 2018, 17(4): 5088-5094.
[4] Cui L, Feng L, Zhang Z H, et al. The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation[J]. International Immunopharmacology, 2014, 23(1): 294-303.
[5] Lin M, Li L, Zhang Y, et al. Baicalin ameliorates H2O2 induced cytotoxicity in HK-2 cells through the inhibition of ER stress and the activation of Nrf2 signaling[J]. International journal of molecular sciences, 2014, 15(7): 12507-12522.
[6] Lin M, Li L, Li L, et al. The protective effect of baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis[J]. BMC complementary and alternative medicine, 2014, 14: 1-9.
[7] Ma L, Wu F, Shao Q, et al. Baicalin alleviates oxidative stress and inflammation in diabetic nephropathy via Nrf2 and MAPK signaling pathway[J]. Drug design, development and therapy, 2021: 3207-3221.
| 세포 실험 [1]: | |
세포 라인 | RAW264.7세포 |
제조 방법 | 세포는 0.005-0.5nM의 Baicalin과 LPS (1 μg/ml)로 처리되었습니다. 각 웰에 10μl (5mg/ml)의 MTT 용액을 추가하고 4시간 동안 배양했습니다. 배양 끝난 후 매개의 제거 후 100μl의 DMSO를 추가하여 10분간 더 배양했습니다. |
반응 조건 | 0.005-0.5nM; 4시간 동안 |
응용 분야 | 1μg/ml LPS에 노출은 세포 증식을 현저히 증가시켰고 Baicalin은 LPS 유도된 RAW264.7 세포 증식에 억제 효과가 있었습니다. |
| 동물 실험 [2]: | |
동물 모형 | 수컷 Wistar 쥐 |
제조 방법 | 쥐는 무작위로 6마리씩 5개 그룹으로 나뉘어졌고 왼쪽 신장동맥을 45분 동안 클램프하고 오른쪽 신장 제거하여 신장 뇌혈 재관류 정상 (IRI)을 유도했습니다. 식염수 처리된 동물은 신장 클램프 30분 전에 1mL 0.9%의 멸균된 NaCl을 복강 주입했습니다. Baicalin 처리된 쥐는 신장 클램프 30분 전에 Baicalin을 멸균된 식염수에 희석하여 1, 10, 또는 100 mg/kg 체중당 복강 주입했습니다. |
제형 | 1, 10또는 100 mg/kg; i.p. |
응용 분야 | Baicalin 치료는 산화 스트레스를 감소시키고 조직학적 손상을 줄였고 신장 기능을 개선하고 염증 반응과 튜부 소양을 억제했습니다. |
References: | |
| Cas No. | 21967-41-9 | SDF | |
| Synonyms | Baicalein 7-glucuronide | ||
| Chemical Name | (2S,3S,4S,5R,6S)-6-(5,6-dihydroxy-4-oxo-2-phenylchromen-7-yl)oxy-3,4,5-trihydroxyoxane-2-carboxylic acid | ||
| Canonical SMILES | C1=CC=C(C=C1)C2=CC(=O)C3=C(C(=C(C=C3O2)OC4C(C(C(C(O4)C(=O)O)O)O)O)O)O | ||
| Formula | C21H18O11 | M.Wt | 446.37 |
| Solubility | DMSO: ≥ 100 mg/mL (224.03 mM） | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2403 mL | 11.2015 mL | 22.4029 mL |
| 5 mM | 448.1 μL | 2.2403 mL | 4.4806 mL |
| 10 mM | 224 μL | 1.1201 mL | 2.2403 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
Baicalin prevented ΔΨm loss and mitochondrial ROS generation that BLM-induced in MLE-12, 5-HD blocked the protective effect. (A) MitoTracker (red) probe indicated the change of mitochondrial membrane potential changes in MLE-12 cells.
For experiments, cells were seeded in plates and grown to 60%− 70% confluency before they were treated with 5-Hydroxydecanoate sodium was (100μM) for 30min, diazoxide (100μM) or baicalin (90μM，GLPBIO, USA) for 30min.
Toxicology (2023): 153638. PMID: 37783230 IF: 4.5003 -
Related Biological Data
Baicalin inhibited expression of FTH1 in OSCC cells. (I, J) Western blot analysis of FTH1 expression changes after overexpression and baicalin treatment.
The CCK8 assay was used to detect the cell viability of Cal27 and SCC25 cells treated with baicalin (GLPBIO, USA) at 0, 5, 10, 20, 4, and 60μM for 24h.
The Journal of Gene Medicine 26.2 (2024): e3669. PMID: 38380717 IF: 3.5
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)