Cholesterol esterase |
Catalog No.GC19870
One-Click Copy Product Info
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Cholesterol esterase is a hydrolase that catalyzes the conversion of ingested cholesterol ester to free cholesterol and free fatty acid.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9026-00-0
Sample solution is provided at 25 µL, 10mM.
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Cholesterol esterase is a hydrolase that catalyzes the conversion of ingested cholesterol ester to free cholesterol and free fatty acid [1]. Cholesterol esterase exhibits proatherogenic properties by facilitating the conversion of the larger and less atherogenic lipoprotein particles to smaller and more atherogenic lipoprotein subspecies [2]. Cholesterol esterase can be covalently immobilized on the polyaniline film to develop a biosensor for cholesterol measurement[3].
In vitro, Cholesterol esterase (100mU/ml) treatment for 3 hours promotes the proliferation and migration of human umbilical vein endothelial cells (HUVECs) [4].
References:
[1] Heidrich J E, Contos L M, Hunsaker L A, et al. Inhibition of pancreatic cholesterol esterase reduces cholesterol absorption in the hamster[J]. BMC pharmacology, 2004, 4(1): 5.
[2] Li B, Zhou B, Lu H, et al. Phosphaisocoumarins as a new class of potent inhibitors for pancreatic cholesterol esterase[J]. European journal of medicinal chemistry, 2010, 45(5): 1955-1963.
[3] Singh S, Solanki P R, Pandey M K, et al. Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor[J]. Analytica Chimica Acta, 2006, 568(1-2): 126-132.
[4] Rebaï O, Le Petit-Thevenin J, Bruneau N, et al. In vitro angiogenic effects of pancreatic bile salt-dependent lipase[J]. Arteriosclerosis, thrombosis, and vascular biology, 2005, 25(2): 359-364.
| Cell experiment [1]: | |
Cell lines | Human umbilical vein endothelial cells (HUVECs) |
Preparation Method | HUVECs were incubated with EBM-2 medium supplemented with 2% fetal calf serum (FCS) in a humidified atmosphere at 37°C with 5% CO2. HUVEC migration was determined using a 24-well chemotaxicell chamber with polycarbonate filter (8-μm pore size). HUVECs (2×104 cells) in serum-free medium (100μl) were applied in the upper compartment of the chamber. The medium containing Cholesterol esterase (100mU/ml) was added to the lower compartment of the chamber and incubated (37°C; 3 hours). After removing Cholesterol esterase and a further incubation (37°C; 24 hours), the non-invading cells on the upper surface of the filter were completely wiped off with a cotton swab and filters were fixed (70% ethanol in PBS) and stained (hematoxylin). The number of migrated cells was counted in 10 random fields at 200× magnification under the microscope. |
Reaction Conditions | 100mU/ml; 3h |
Applications | Cholesterol esterase treatment significantly promoted cell migration of HUVECs. |
References: | |
| Cas No. | 9026-00-0 | SDF | |
| Formula | M.Wt | ||
| Solubility | H2O : ≥ 50 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: 42 u/mg solid Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)