7,4'-Dihydroxyflavone (Synonyms: 7,4'-DHF) |
Catalog No.GC35185
One-Click Copy Product Info
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7,4'-Dihydroxyflavone은Glycyrrhiza uralensis (감초)로부터 분리한 경구 활성 플라보노이드로 eotaxin/CCL11 및 카르보닐 레덕타제 1(CBR1) 억제제로 작용한다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2196-14-7
Sample solution is provided at 25 µL, 10mM.
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7,4'-Dihydroxyflavone은Glycyrrhiza uralensis (감초)로부터 분리한 경구 활성 플라보노이드로 eotaxin/CCL11 및 카르보닐 레덕타제 1(CBR1) 억제제로 작용한다. 이 물질은 eotaxin 생성을 지속적으로 억제하고 덱사메타손(Dex) 중단 후 나타나는 eotaxin rebound 효과를 방지한다[2]. 게다가 NF-κB·STAT6·HDAC2 신호전달을 조절해서 MUC5AC 유전자 발현과 점액 분비및 생성을 억제한다[3].
인간 폐 섬유아세포-1에서 7,4'-Dihydroxyflavone(10 μM, 24–72 h)는 Dex LT 배양에 의한 p-STAT6 증가를 억제하고 HDAC2 발현을 감소시켰다[2]. NCI-H292 세포에서 10 μM 7,4'-Dihydroxyflavone은 PMA에 의해 유도된 MUC5AC 생성을 현저하게 억제하였다[3].
오발부민 민감화/재도전 쥐 모델에서 7,4'-dihydroxyflavone은 체내 MUC5AC 분비를 억제하였다. 6 µg/mL 농도로 4주간 처리한 결과는 오발부민 민감화/재도전 쥐의 기관지폐포세척액(BAL) 내 MUC5AC 단백 수준을 현저하게 감소시켰다[3].
References:
[1]. Liu C, Yang N, Robalino R P, et al. 7, 4'-Dihydroxyflavone isolated from Glycyrrhiza uralensis a constituent of ASHMITM prevents dexamethasone enhancement of Eotaxin-1 secretion by human lung fibroblasts[J]. Journal of Allergy and Clinical Immunology, 2014, 133(2): AB142.
[2]. Liu ChangDa L C D, Yang Nan Y N, Chen XiaoKe C X K, et al. The flavonoid 7, 4′-dihydroxyflavone prevents dexamethasone paradoxical adverse effect on eotaxin production by human fibroblasts[J]. 2017.
[3]. Liu C, Weir D, Chakrapani S, et al. The Flavonoid 7, 4'-Dihydroxyflavone Inhibits Human Airway Epithelial Cells MUC5AC Mucin Production Via Regulation of NF-êb, STAT6 and HDAC2[J]. Journal
| 세포 실험 [1]: | |
세포 라인 | NCI-H292 세포 |
제조 방법 | 농도 2×10⁵ 세포/mL의 NCI-H292 세포를 96-엘 조직배양판에 100 µL씩 접종해서 24 시간 동안 부착시켰습니다. 이후 200 µL PBS로 3회 세척하고 다음과 같이 처리하였습니다: 무처리, 10 ng/mL PMA 단독 처리 또는 10 ng/mL PMA에 무혈청 RPMI 배지에서 다양한 농도의 7,4'-Dihydroxyflavone을 병용 처리하였습니다. |
반응 조건 | 10-50μM; 24 시간 동안 |
응용 분야 | 10 µM 7,4'-Dihydroxyflavone은 H292 세포에서 PMA에 의해 유도된 MUC5AC 생성을 현저하게 억제하였습니다. |
| 동물 실험 [2]: | |
동물 모형 | OVA 면역 자극/자극 치료와 7,4'-Dihydroxyflavone 치료를 시행된 쥐 모델 |
제조 방법 | 알레르기성 천식 모델 구축을 위해 암컷 BALB/c 쥐에게 2주간 매주 200 mg OVA와 2 mg 알룸(0.4 mL인산완충식염수에 흡착)을 복강내 주입해서 민감화시켰습니다. 최종 민감화 후 14, 21, 28, 45, 46일차에 각각 0.05 mL PBS에 녹인 100 mg OVA를 기관내(i.t.)로 5회 도전하였습니다. 첫 기관내 도전 24시간 후부터 3마리를 선택해서 1 mL 0.1 % DMSO 수용액에 녹인 6 µg 7,4'-Dihydroxyflavone을 1개월간 하루 2회 경구 투여하였습니다. |
제형 | 6µg/mL; po; bid; 1 개월 동안 |
응용 분야 | 7,4'-Dihydroxyflavone은 체내에서 MUC5AC 분비를 억제합니다; 6 µg/mL 농도로 4주간 경구 투여한 결과는 OVA 민감화/도전 쥐의 기관지폐포세척액(BAL) 내 MUC5AC 단백 수준이 현저하게 감소하였습니다. |
References: | |
| Cas No. | 2196-14-7 | SDF | |
| Synonyms | 7,4'-DHF | ||
| Canonical SMILES | O=C1C=C(C2=CC=C(O)C=C2)OC3=CC(O)=CC=C13 | ||
| Formula | C15H10O4 | M.Wt | 254.24 |
| Solubility | DMSO: ≥ 250 mg/mL (983.32 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9333 mL | 19.6665 mL | 39.3329 mL |
| 5 mM | 786.7 μL | 3.9333 mL | 7.8666 mL |
| 10 mM | 393.3 μL | 1.9666 mL | 3.9333 mL |
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Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)