7,4'-Dihydroxyflavone (Synonyms: 7,4'-DHF) |
Catalog No.GC35185
One-Click Copy Product Info
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7,4'-Dihydroxyflavone ,an orally active flavonoid isolated from Glycyrrhiza uralensis (licorice), functions as an inhibitor of eotaxin/CCL11 and carbonyl reductase 1 (CBR1) .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2196-14-7
Sample solution is provided at 25 µL, 10mM.
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7,4'-Dihydroxyflavone ,an orally active flavonoid isolated from Glycyrrhiza uralensis (licorice), functions as an inhibitor of eotaxin/CCL11 and carbonyl reductase 1 (CBR1) [1]. It persistently suppresses eotaxin production and prevents the rebound effect of dexamethasone (Dex) on eotaxin generation [2]. Furthermore,7,4'-Dihydroxyflavone inhibits MUC5AC gene expression, mucus production, and secretion by modulating the NF-κB, STAT6, and HDAC2 signaling pathways[3].
In human lung fibroblast-1 cells, 7,4'-Dihydroxyflavone (10μM; 24-72h) inhibited the enhancement of p-STAT6 in Dex LT culture and suppressed the expression of HDAC2. [2]. In NCI-H292 cells, 10µM of 7,4'-Dihydroxyflavone significantly inhibited PMA-induced MUC5AC production [3].
In an ovalbumin-sensitized/challenged mouse model, 7,4'-dihydroxyflavone inhibited MUC5AC secretion in vivo. Treatment with 6µg/mL of 7,4'-dihydroxyflavone for 4 weeks significantly reduced the levels of MUC5AC protein in the bronchoalveolar lavage fluid (BAL) of ovalbumin-sensitized/challenged mice [3].
References:
[1]. Liu C, Yang N, Robalino R P, et al. 7, 4'-Dihydroxyflavone isolated from Glycyrrhiza uralensis a constituent of ASHMITM prevents dexamethasone enhancement of Eotaxin-1 secretion by human lung fibroblasts[J]. Journal of Allergy and Clinical Immunology, 2014, 133(2): AB142.
[2]. Liu ChangDa L C D, Yang Nan Y N, Chen XiaoKe C X K, et al. The flavonoid 7, 4′-dihydroxyflavone prevents dexamethasone paradoxical adverse effect on eotaxin production by human fibroblasts[J]. 2017.
[3]. Liu C, Weir D, Chakrapani S, et al. The Flavonoid 7, 4'-Dihydroxyflavone Inhibits Human Airway Epithelial Cells MUC5AC Mucin Production Via Regulation of NF-êb, STAT6 and HDAC2[J]. Journal
| Cell experiment [1]: | |
Cell lines | NCI-H292 cells |
Preparation Method | 100µL of NCI-H292 cells at a concentration of 2x10⁵ cells/mL were added to a 96-well tissue culture plate and allowed to adhere. After 24 hours, the cells were washed three times with 200µL of PBS, and then treated as follows: no treatment, treatment with 10ng/mL PMA alone, or treatment with 10ng/mL PMA plus different concentrations of 7,4'-Dihydroxyflavone in serum-free RPMI medium. |
Reaction Conditions | 10-50μM; 24h |
Applications | 10µM of 7,4'-Dihydroxyflavone significantly inhibited PMA-induced MUC5AC production in H292 cells. |
| Animal experiment [1]: | |
Animal models | OVA sensitization/challenge and 7,4'-Dihydroxyflavone treatment in murine model |
Preparation Method | To establish an allergic asthma model, female BALB/c mice were sensitized by weekly intraperitoneal injection of 200mg OVA and 2mg alum (adsorbed in 0.4mL phosphate-buffered saline) for two weeks. After the last sensitization, five intratracheal (i.t.) challenges were performed on days 14, 21, 28, 45, and 46, each time injecting 100mg OVA dissolved in 0.05mL phosphate-buffered saline. Three mice, starting 24 hours after the first intratracheal challenge, were orally administered 6µg of 7,4'-Dihydroxyflavone dissolved in 1mL of 0.1% DMSO aqueous solution twice daily for one month. |
Dosage form | 6µg/mL; po; bid; 1 month |
Applications | 7,4'-Dihydroxyflavone inhibits MUC5AC secretion in vivo. After treatment with 6µg/mL of 7,4'-Dihydroxyflavone for 4 weeks, the MUC5AC protein levels in the bronchoalveolar lavage fluid (BAL) of OVA-sensitized/challenged mice were significantly reduced. |
References: | |
| Cas No. | 2196-14-7 | SDF | |
| Synonyms | 7,4'-DHF | ||
| Canonical SMILES | O=C1C=C(C2=CC=C(O)C=C2)OC3=CC(O)=CC=C13 | ||
| Formula | C15H10O4 | M.Wt | 254.24 |
| Solubility | DMSO: ≥ 250 mg/mL (983.32 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9333 mL | 19.6665 mL | 39.3329 mL |
| 5 mM | 786.7 μL | 3.9333 mL | 7.8666 mL |
| 10 mM | 393.3 μL | 1.9666 mL | 3.9333 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
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Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)