Methoxy-X04

This plan only provides a guide, please modify it to meet your specific needs.

1. Prepare dyeing solution

Solvent: 10% DMSO, 45% propylene glycol and 45% phosphate buffered saline (pH7.5, 0.01M, 5mg/ml)

(1) Use a microbalance to weigh 5 mg of Methoxy-X04, dissolve Methoxy-X04 in DMSO under a fume hood and stir until a clear green solution is obtained;

(2) Add propylene glycol and phosphate buffered saline in sequence, stirring each time;

(3) Place the solution on a rotator and stir at 4°C until it becomes a yellow-green emulsion;

2. Animal injection: The experimental animals are mice as an example. Methoxy-X04 is administered via intraperitoneal injection at a dose of 10 mg/kg.

3. Tissue frozen section, recommended sample thickness: 40-50 µm;

4. Examine the sections under a fluorescence microscope to identify areas containing methoxy-X04-labeled Aβ plaques. NOTE: Methoxy-X04 can be easily observed using a fluorescent ultraviolet (UV) filter (excitation wavelength 340 - 380 nm).

Precautions:

1) Fluorescent dyes all have quenching problems. Please avoid light as much as possible to slow down fluorescence quenching.

2) For your safety and health, please wear a lab coat and disposable gloves.

References:

[1]. Kanchan Bisht,et,al. Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques. 2016 Jun 1:(112):54060. doi: 10.3791/54060.

[2]. Marcin Sadowski,et,al. Targeting prion amyloid deposits in vivo. 2004 Jul;63(7):775-84. doi: 10.1093/jnen/63.7.775.