2',3'-Dideoxyadenosine 5'-triphosphate (lithium salt) (Synonyms: ddATP) |
Katalog-Nr.GC52501 |
An inhibitor of reverse transcriptases and DNA polymerases
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
2',3'-Dideoxyadenosine 5'-triphosphate (ddATP) is an in vitro inhibitor of reverse transcriptases from retroviruses, including HIV-1 and visna (Kis = 20 and 37 nM, respectively).1,2,3 It also blocks, in vitro, mammalian and bacterial DNA polymerases.4,5 ddATP, produced intracellularly by the phosphorylation of exogenously supplied 2',3'-dideoxyadenosine, competes with dATP, resulting in chain termination.4,5 Because of this activity, dideoxynucleoside 5'-triphosphates, including ddATP, are used to terminate chain extension produced by the Taq polymerase used in polymerase chain reactions.6 It is also an antagonist of the purinergic receptor P2X2/3 (pIC50 = 6.5).7
1.Boyle, N.A., Rajwanshi, V.K., Prhavc, M., et al.Synthesis of 2',3'-dideoxynucleoside 5'-α-P-borano-β,γ-(difluoromethylene)triphosphates and their inhibition of HIV-1 reverse transcriptaseJ. Med. Chem.48(7)2695-2700(2005) 2.Frank, K.B., McKernan, P.A., Smith, R.A., et al.Visna virus as an in vitro model for human immunodeficiency virus and inhibition by ribavirin, phosphonoformate, and 2',3'-dideoxynucleosidesAntimicrob. Agents Chemother.31(9)1369-1374(1987) 3.Ueno, T., Shirasaka, T., and Mitsuya, H.Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase resistant to multiple 2',3'-dideoxynucleoside 5'-triphosphatesJ. Biol. Chem.270(40)23605-23611(1995) 4.Toji, L., and Cohen, S.S.The enzymatic termination of polydeoxynucleotides by 2',3'-dideoxyadenosine triphosphateProc. Natl. Acad. Sci. USA63(3)871-877(1969) 5.Yagura, T., Kozu, T., and Seno, T.Mouse DNA polymerase accompanied by a novel RNA polymerase activity: Purification and partial characterizationJ. Biochem.91(2)607-618(1982) 6.Li, Y., Mitaxov, V., and Waksman, G.Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporationProc. Natl. Acad. Sci. USA96(17)9491-9496(1999) 7.Jarvis, M.F., Bianchi, B., Uchic, J.T., et al.[3H]A-317491, a novel high-affinity non-nucleotide antagonist that specifically labels human P2X2/3 and P2X3 receptorsJ. Pharmacol. Exp. Ther.310(1)407-416(2004)
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(Based on Reviews and 30 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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